Members of this family are distantly related to CofD, the enzyme LPPG:FO 2-phospho-L-lactate transferase, involved in coenzyme F420 biosynthesis. This family appears to belong to a biosynthesis cassette of unknown function.
Members of this family are ATP-grasp family enzymes related to a number of characterised glutamate ligases, including the ribosomal protein S6 modification enzyme RimK. This group belongs to a conserved gene neighbourhood that also features an HPr kinase-related protein. We assign this system the initial designation GAK, for Grasp (this ATP-grasp family enzyme), Amphipathic (for the member of the domain entry , designated Amphi-Trp), and Kinase, for the HPr-kinase homologue .