This entry represents Vps53 from eukaryotes. Vps53 is part of the of the GARP (Golgi-associated retrograde protein) complex involved in retrograde transport from early and late endosomes to late Golgi [, , ]. Mutations in the human Vps53 gene cause pontocerebellar hypoplasia 2E (PCH2E), a neurodegenerative disorder characterised by progressive cerebello-cerebral atrophy, profound mental retardation, progressive microcephaly, spasticity, and early-onset epilepsy [].
Vps52 complexes with Vps53 and Vps54 to form the Golgi-associated retrograde protein (GARP) complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network, regulating membrane trafficking events [, , ]. It is also part of the EARP (Endosome-Associated Recycling Protein) complex, involved in endocytic recycling [].
This superfamily represents the C-terminal domain of vacuolar protein sorting-associated protein 53 (Vps53), which is part of the of the GARP (Golgi-associated retrograde protein) complex involved in retrograde transport from early and late endosomes to late Golgi [, , ]. Budding yeast Vps53 is involved in retrograde transport from early and late endosomes to late Golgi by linking the vesicle through the t-SNARE Tgl1 to the Golgi, leading to the membrane fusion between late Golgi and endosomal vesicles [].
The VFT tethering complex (also known as GARP complex, Golgi associated retrograde protein complex, Vps53 tethering complex) is a conserved eukaryotic docking complex which is involved in recycling of proteins from endosomes to the late Golgi. Vps51 (also known as Ang2 or Vps67) is a subunit of VFT and interacts with the SNARE Tlg1 []. It is also part of the endosome-associated recycling protein (EARP) complex which is structurally related to GARP complex [].
Vps53 complexes with Vps52 and Vps54 to form a multi-subunit complex called Golgi-Associated Retrograde Protein complex (GARP) involved in retrograde transport from early and late endosomes to late Golgi [, , ]. They are members of the CATCHR (complexes associated with tethering containing helical rods) which includes the exocyst, COG, GARP, and DSL1 complexes which have structural and functional similarities. This is the N-terminal coiled-coil domain [].
This entry represents the C-terminal domain of fungal vacuolar protein sorting-associated protein 53 (Vps53), a component of the Golgi-associated retrograde complex (GARP). Budding yeast Vps53 is involved in retrograde transport from early and late endosomes to late Golgi by linking the vesicle through the t-SNARE Tgl1 to the Golgi, leading to the membrane fusion between late Golgi and endosomal vesicles [, ]. This domain has a fold consisting of an α-helical bundle known as the CATCHR fold (complexes associated with tethering containing helical rods) which is shared by proteins of other tethering complexes, namely exocyst, COG and Dsl1 [, ].
