This entry represents a group of DnaJ homologue subfamily C proteins, including GRV2 from Arabidopsis and DNAJC13 (also known as RME-8) from humans. They are involved in endocytosis. GRV2 functions in vesicle trafficking from the multivesicular body/pre-vacuolar compartment to the lytic vacuole []. In Arabidopsis grv2 mutants display enlarged aggregated endosomes, defective vacuole biogenesis and embryogenesis, and reduced gravitropic responses [, , ]. DNAJC13 plays a role in the endosomal transport machinery, in which it coordinates the activity of the WASH complex with the function of the retromer SNX dimer to control endosomal tubulation [].DNAJC13 has been linked to Parkinson disease []. In humans, DNAJC13 localizes to early endosomal compartments via an N-terminal PI(3)P targeting motif and binds the co-chaperone Heat shock cognate-70 (Hsc70) through the central J domain, whereas the remainder interacts with the BAR-domain containing sorting nexin 1 (SNX1) protein and the FAM21 subunit of the WASH complex[].
This is the N-terminal domain of DNAJC13/GVR2, which is required for membrane association and interaction with FAM21 tail domain []. It contains critical residues mediating phosphatidylinositol 3-phosphate (PI(3)P) binding, required for its association with endosomes [].This entry represents a group of DnaJ homologue subfamily C proteins, including GRV2 from Arabidopsis and DNAJC13 (also known as RME-8) from humans. They are involved in endocytosis. GRV2 functions in vesicle trafficking from the multivesicular body/pre-vacuolar compartment to the lytic vacuole []. In Arabidopsis grv2 mutants display enlarged aggregated endosomes, defective vacuole biogenesis and embryogenesis, and reduced gravitropic responses [, , ]. DNAJC13 plays a role in the endosomal transport machinery, in which it coordinates the activity of the WASH complex with the function of the retromer SNX dimer to control endosomal tubulation [].DNAJC13 has been linked to Parkinson disease []. In humans, DNAJC13 localizes to early endosomal compartments via an N-terminal PI(3)P targeting motif and binds the co-chaperone Heat shock cognate-70 (Hsc70) through the central J domain, whereas the remainder interacts with the BAR-domain containing sorting nexin 1 (SNX1) protein and the FAM21 subunit of the WASH complex[].
This domain is found in bacteria, archaea and eukaryotes, and is approximately 50 amino acids in length. It contains an evolutionary conserved signature W-X-Y-X6-11-GPF-X4-M-X2-W-X3-GYF, the site of interaction with proline-rich peptides. Proteins containing this domain include RME-8 (Required for receptor-mediated endocytosis 8), a DNAJC13 protein. RME-8 was first identified as a protein that is required for endocytosis in Caenorhabditis elegans. It coordinates the activity of the WASH complex with the function of the retromer SNX dimer to control endosomal tubulation []. Proteins containing this domain also include Arabidopsis trithorax-related3 (Atxr3) and Tic56. Atxr3 is the major enzyme responsible for H3K4me3, which is critical for regulating gene expression and plant development []. Tic56 is an essential subunit of a 1-MDa protein complex at the inner chloroplast envelope membrane []. Tic56 also plays important roles in rRNA processing and chloroplast ribosome assembly [].
