Members of this protein family are DltB, part of a four-gene operon for D-alanyl-lipoteichoic acid biosynthesis that is present in the vast majority of low-GC Gram-positive organisms []. It is an O-acyltransferase that catalyzes D-alanylation of both teichoic acid and lipoteichoic acid (LTA) []. D-alanylation of LTA plays an important role in modulating the properties of the cell wall in Gram-positive bacteria, influencing the net charge of the cell wall [].