TTLL10 is a polyglycylase, which modifies both tubulin and non-tubulin proteins, generating side chains of glycine on the γ-carboxyl groups of specific glutamate residues of target proteins []. It polyglycylates alpha tubulin and beta tubulin, but is not able to initiate glycylation and only has activity toward monoglycylated tubulin []. It has the ability to polyglycylate non-tubulin proteins such as NAP1; in this case it can initiate glycylation and does not require preliminary monoglycylation by another glycylase [].This family also includes , which is inactive [].
