This entry include a group of proteins involved in chromatin remodelling, including Vps72 (vacuolar protein sorting-associated protein 72) from budding yeasts. Vps72 is a Htz1-binding component of the SWR1 complex, which is required for the incorporation of the histone variant H2AZ into chromatin []. It is also required for vacuolar protein sorting in budding yeasts [].The Vps72 homologue from animals, YL-1, is a deposition-and-exchange histone chaperone specific for H2AZ1, specifically chaperones H2AZ1 and deposits it into nucleosomes. It is component of the SRCAP and Tip60 complexes, and mediates the ATP-dependent exchange of histone H2AZ1/H2B dimers for nucleosomal H2A/H2B, leading to transcriptional regulation of selected genes by chromatin remodeling [, ].
This entry include a group of proteins involved in chromatin remodelling, including Vps72 (vacuolar protein sorting-associated protein 72) from budding yeasts. Vps72 is a Htz1-binding component of the SWR1 complex, which is required for the incorporation of the histone variant H2AZ into chromatin []. It is also required for vacuolar protein sorting in budding yeasts [].The Vps72 homologue from animals, YL-1, is a deposition-and-exchangehistone chaperone specific for H2AZ1, specifically chaperones H2AZ1 and deposits it into nucleosomes. It is component of the SRCAP and Tip60 complexes, and mediates the ATP-dependent exchange of histone H2AZ1/H2B dimers for nucleosomal H2A/H2B, leading to transcriptional regulation of selected genes by chromatin remodeling [, ].This is the N-terminal region from Vps72/YL1, which covers the aspartate- and glutamate-rich N-terminal domain and the central DNA-binding domain [, ].
This entry consists of the SWR1-complex protein 4 (Swc4) from yeast, also known as EAF2, and its mammalian homologue DNA methyltransferase 1-associated protein 1 (Dmap1) []. They are components of the NuA4 histone acetyltransferase (HAT) complex, which is highly conserved in eukaryotes. NuA4 acetylates the nucleosomal histones H4 and H2A and plays primary roles in transcription, DNA repair, and cell cycle control [, ].The subunits of the NuA4 complex are shared by the ATP-dependent chromatin-remodeling SWR1 complex in yeast and its human orthologue, Snf 2-related CREB-binding protein (CBP) activator protein (SRCAP) complex. The mammalian NuA4 complex includes the SRCAP complex, and thus combines the functions of the yeast NuA4 and Swr1 complexes []. The SWR1/SRCAP complex mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling [, , ]. Dmap1 was originally identified as an interacting molecule with DNA methyltransferase 1 (DNMT1) and plays a crucial role in DNA repair. It is indispensable for the maintenance of chromosomal integrity and might function as a tumor suppressor [].
