In budding yeasts, Golgi to ER traffic protein 1 (Get1) is a component of the Golgi to ER traffic (GET) complex, which is composed of Get1, Get2 and Get3. The GET complex mediates posttranslational insertion of newly synthesised tail-anchored (TA) proteins to the endoplasmic reticulum (ER) membrane.The GET complex is composed of the homodimeric Get3 ATPase and its heterooligomeric receptor, Get1/2. Get1 stabilises an open dimer conformation of Get3 [].In metazoa, the homologue to Get1 is known as tail-anchored protein insertion receptor WRB, which is a receptor for ASNA1/TRC40 (Get3 in yeast) [].
The Golgi to ER traffic (GET) complex is composed of Get1, Get2 and Get3. The complex is involved in the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum []. Get1 and Get2 form a transmembrane complex and interact with Get3, an ATPase which recognises and selectively binds the transmembrane domain of TA proteins in the cytosol [, ].
The Golgi to ER traffic (GET) complex is composed of Get1, Get2 and Get3. The complex is involved in the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum []. Get1 and Get2 form a transmembrane complex and interact with Get3, an ATPase which recognises and selectively binds the transmembrane domain of TA proteins in the cytosol [, ].
This entry represents the GET complex subunit Get2 and its homologue, sif1 from Schizosaccharomyces pombe. The Golgi to ER traffic (GET) complex is composed of Get1, Get2 and Get3. The complex is involved in the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum []. Get1 and Get2 form a transmembrane complex and interact with Get3, an ATPase which recognises and selectively binds the transmembrane domain of TA proteins in the cytosol [, ]. In Schizosaccharomyces pombe, sif1 interacts with Kms1 and Sad1, which are constitutive membrane-bound components of the SPB (spindle pole body) that interact with each other [].
This entry represents the Guided entry of tail-anchored proteins factor CAMLG (also known as Calcium signal-modulating cyclophilin ligand CAML) which is required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum [, , ]. Together with GET1/WRB, acts as a membrane receptor for soluble GET3/TRC40, which recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol [, , ]. It is required for the stability of GET1 []. CAMLG also stimulates calcium signaling in T cells through its involvement in elevation of intracellular calcium [], and it is essential for the survival of peripheral follicular B cells. Although CAML shows no sequence similarity to yeast Get2, they share similar biochemical properties and topology [], thus resulting in the Get2 homologue in mammals, playing the same essential role in the membrane insertion as that of Get2. It also shows similarity with ER membrane protein complex subunit 6 (EMC6) [].
