This entry represents the RNA recognition motif 3 (RRM3) of PUF60 (also known as FIR). PUF60 is an essential splicing factor that functions as a poly-U RNA-binding protein required to reconstitute splicing in depleted nuclear extracts. Its function is enhanced through interaction with U2 auxiliary factor U2AF65 []. PUF60 also controls human c-myc gene expression by binding and inhibiting the transcription factor far upstream sequence element (FUSE)-binding-protein (FBP), an activator of c-myc promoters []. PUF60 contains two central RNA recognition motifs (RRMs) and a C-terminal U2AF (U2 auxiliary factor) homology motifs (UHM) that harbours another RRM and binds to tryptophan-containing linear peptide motifs (UHM ligand motifs, ULMs) in several nuclear proteins [].
This entry represents the RNA recognition motif 1 (RRM1) of PUF60 (also known as FIR). PUF60 is an essential splicing factor that functions as a poly-U RNA-binding protein required to reconstitute splicing in depleted nuclear extracts. Its function is enhanced through interaction with U2 auxiliary factor U2AF65 []. PUF60 also controls human c-myc gene expression by binding and inhibiting the transcription factor far upstream sequence element (FUSE)-binding-protein (FBP), an activator of c-myc promoters []. It has anti-inflammatory properties, playing a role in the host response []. PUF60 contains two central RNA recognition motifs (RRMs) and a C-terminal U2AF (U2 auxiliary factor) homology motifs (UHM) that harbours another RRM and binds to tryptophan-containing linear peptide motifs (UHM ligand motifs, ULMs) in several nuclear proteins [].
This entry represents the RNA recognition motif 2 (RRM2) of PUF60 (also known as FIR). PUF60 is an essential splicing factor that functions as a poly-U RNA-binding protein required to reconstitute splicing in depleted nuclear extracts. Its function is enhanced through interaction with U2 auxiliary factor U2AF65 []. PUF60 also controls human c-myc gene expression by binding and inhibiting the transcription factor far upstream sequence element (FUSE)-binding-protein (FBP), an activator of c-myc promoters []. It has anti-inflammatory properties, playing a role in the host response []. PUF60 contains two central RNA recognition motifs (RRMs) and a C-terminal U2AF (U2 auxiliary factor) homologymotifs (UHM) that harbours another RRM and binds to tryptophan-containing linear peptide motifs (UHM ligand motifs, ULMs) in several nuclear proteins [].
Proteins in this family are DNA- and/or RNA-binding proteins involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation [, ], containing three RNA recognition motifs [, , ]. In the case of PUF60, in complex with p54, and in the presence of U2AF, it facilitates association of U2 snRNP with pre-mRNA []. Poly(U)-binding-splicing factor rnp-6 from C.elegans is an homologue of mammalian PUF60 and it ensures the correct splicing of genes involved in immunity to promote longevity in response to infection by pathogenic bacteria such as S.aureus [].
