This entry includes Sld2 from budding yeasts and Drc1 from fission yeasts. Sld2 is required for the initiation of DNA replication. Its association with DNA is required for the assembly of the Cdc45-Mcm2-7-GINS (CMG) replicative helicase complex in S phase []. Sld2 can be regulated by S-Cdk-dependent phosphorylation []. Similarly, Drc1 is regulated by Cdk phosphorylation and is required for DNA replication []. Drc1 interacts with Cut5 and plays a role in the loading of Cut5 onto origins of replication [].
DRC1 is a key component of the nexin-dynein regulatory complex (N-DRC), essential for N-DRC integrity. It is required for the assembly and regulation of specific classes of inner dynein arm motors. It may also function to restrict dynein-driven microtubule sliding, thus aiding in the generation of ciliary bending []. Mutations of DRC1 gene cause Ciliary dyskinesia, primary, 21 (CILD21), which is a disorder characterised by abnormalities of motile cilia []. DRC2, also known as CCDC65, is an essential component of the nexin-dynein regulatory complex (N-DRC)[]. DRC2 is necessary for the co-assembly of DRC2 and DRC1 to form the base plate of N-DRC.
Genome duplication is precisely regulated by cyclin-dependent kinases CDKs, which bring about the onset of S phase by activating replication origins and then prevent relicensing of origins until mitosis is completed. The optimum sequence motif for CDK phosphorylation is S/T-P-K/R-K/R, and Drc1-Sld2 is found to have at least 11 potential phosphorylation sites. Drc1 is required for DNA synthesis and S-M replication checkpoint control. Drc1 associates with Cdc2 and is phosphorylated at the onset of S phase when Cdc2 is activated. Thus Cdc2 promotes DNA replication by phosphorylating Drc1 and regulating its association with Cut5 []. Sld2 and Sld3 represent the minimal set of S-CDK substrates required for DNA replication [].This entry also includes ATP-dependent DNA helicase Q4, which may be involved in chromosome segregation and has been associated with various diseases [, ].
This entry represents the C-terminal domain of dynein regulatory complex protein 1 (DRC1, also known as CCDC164). DRC1 is a key component of the nexin-dynein regulatory complex (N-DRC), essential for N-DRC integrity. It is required for the assembly and regulation of specific classes of inner dynein arm motors. It may also function to restrict dynein-driven microtubule sliding, thus aiding in the generation of ciliary bending []. Mutations of DRC1 gene cause Ciliary dyskinesia, primary, 21 (CILD21), which is a disorder characterised by abnormalities of motile cilia [].
This entry represents the N-terminal domain of dynein regulatory complex protein 1/2 (DRC1/2). DRC1 is a key component of the nexin-dynein regulatory complex (N-DRC), essential for N-DRC integrity. It is required for the assembly and regulation of specific classes of inner dynein arm motors. It may also function to restrict dynein-driven microtubule sliding, thus aiding in the generation of ciliary bending []. Mutations of DRC1 gene cause Ciliary dyskinesia, primary, 21 (CILD21), which is a disorder characterised by abnormalities of motile cilia []. DRC2, also known as CCDC65, is an essential component of the nexin-dynein regulatory complex [].
