This domain is found at the C-terminal region of Nuf2 proteins. This domain was identified as MazG related domain also designated as Designed helical repeat protein 10 (DHR10) []that actually adopts a coiled-coil structure []. Nuf2 is part of the Ndc80 complex, which complex binds to the spindle and is required for chromosome segregation and spindle checkpoint activity [, , , , , , ].
This superfamily represents a domain found at the N terminus of the Kinetochore protein Nuf2, which is part of the Ndc80 complex. This domain fold as a CH domain consisting of a four-helix bundle containing the parallel helices alphaA, alphaC, alphaE, and alphaG, similar to that in Ndc80. These CH domains from Nuf2 and Ndc80 forms the globular rod at one end the complex [, ]. The ability of the Ndc80 complex to bind microtubules resides on the tightly packed CH domains from Nuf2 and Ndc80 []. This complex binds to the spindle and is required for chromosome segregation and spindle checkpoint activity [, , , , , , ].
This entry represents the N-terminal domain of kinetochore protein Nuf2, which is part of the Ndc80 complex. This domain fold as a CH domain consisting of a four-helix bundle containing the parallel helices alphaA, alphaC, alphaE, and alphaG, similar to that in Ndc80. These CH domains from Nuf2 and Ndc80 forms the globular rod at one end the complex [, ]. The ability of the Ndc80 complex to bind microtubules resides on the tightly packed CH domains from Nuf2 and Ndc80 []. This complex binds to the spindle and is required for chromosome segregation and spindle checkpoint activity [, , , , , , ].
Spc24 is a component of the evolutionarily conserved kinetochore-associated Ndc80 complex, which is implicated in several essential outer kinetochore functions, including microtubule binding and control the spindle assembly checkpoint [, ].The Ndc80 complex appears to be a long rod with globular 'heads'. Ndc80 and Nuf2 contribute to one head and to the intervening shaft; Spc24 and Spc25 complete the shaft and contribute to the other head. Spc24 and Spc25 fold tightly together. TheC-terminal globular domains of Spc24 and Spc25 form the 'inner' head, which faces the centromere [].
