RNA cytidine acetyltransferase NAT10 has specificity toward both 18S rRNA and tRNAs and catalyzes the formation of N(4)-acetylcytidine at two positions in 18S rRNA. NAT10 is required for early nucleolar cleavages of precursor rRNA at sites A0, A1 and A2 during 18S rRNA synthesis [, ]. NAT10 also catalyzes the formation of N(4)-acetylcytidine at a single position in serine and leucine tRNAs. The tRNA-binding adapter protein TAN1 is required for full tRNA acetyltransferase activity but not for 18S rRNA acetylation [].Human NAT10 is a lysine acetyltransferase that targets microtubules and histones, and plays an important role in cell division. It is highly expressed in malignant tumors [].
This entry includes bacterial tRNA(Met) cytidine acetyltransferase TmcA, budding yeast Kre33, fission yeast and mammalian N-acetyltransferase 10 (NAT10). They consist of an RNA helicase domain and a GCN5-related N-acetyltransferase (GNAT) domain. TmcA catalyses the formation of N(4)-acetylcytidine (ac4C) at the wobble position of tRNA(Met), by using acetyl-CoA as an acetyl donor and either ATP or GTP []. This modification is thought to ensure precise recognition of the AUG codon by strengthening C-G base-pair interaction and also prevent misrecognition of the near cognate AUA codon []. Kre33 and NAT10 are RNA cytidine acetyltransferases with specificity toward both 18S rRNA and tRNAs [, , ].
This entry represents a domain found in the N terminus of the bacterial tRNA(Met) cytidine acetyltransferase TmcA. TmcA catalyses the formation of N(4)-acetylcytidine (ac4C) at the wobble position of tRNA(Met), by using acetyl-CoA as an acetyl donor and either ATP or GTP []. This modification is thought to ensure precise recognition of the AUG codon by strengthening C-G base-pair interaction and also prevent misrecognition of the near cognate AUA codon []. This domain can also be found in mammalian N-acetyltransferase 10 (NAT10) and fungal protein Kre33. Kre33 and NAT10 are RNA cytosine acetyltransferases with specificity toward both 18S rRNA and tRNAs [, ].
