| Type | Details | Score | 
          
              | Gene | 
              
                | Type: | gene |  
                | Organism: | human |  | • • • • • | 
          
              | Gene | 
              
                | Type: | gene |  
                | Organism: | frog, western clawed |  | • • • • • | 
          
              | Gene |  | • • • • • | 
          
              | Gene | 
              
                | Type: | gene |  
                | Organism: | dog, domestic |  | • • • • • | 
          
              | Gene | 
              
                | Type: | gene |  
                | Organism: | chimpanzee |  | • • • • • | 
          
              | Gene | 
              
                | Type: | gene |  
                | Organism: | cattle |  | • • • • • | 
          
              | Gene | 
              
                | Type: | gene |  
                | Organism: | chicken |  | • • • • • | 
          
              | Gene | 
              
                | Type: | gene |  
                | Organism: | macaque, rhesus |  | • • • • • | 
          
              | Protein Domain | 
              
                | Type: | Family |  
                | Description: | Mitochondrial intermembrane space import and assembly protein 40 (CHCHD4) is a chaperone and catalyses the formation of disulfide bonds in proteins within the mitochondrial intermembrane space (IMS) such as COX17 [], COX19 [], MICU1 []and GFER [].  A precursor of one of these proteins in a reduced state is imported into the mitochondrion where it associates with CHCHD4 forming a transient intermolecular disulfide bridge.  This leads to oxidation of the precursor, formation of an intramolecular disulfide bond and the folding in the IMS [].This entry also includes the uncharacterized protein F42H10.2 from Caenorhabditis elegans. |  | • • • • • | 
          
              | Protein Coding Gene | 
              
                | Type: | protein_coding_gene |  
                | Organism: | mouse, laboratory |  | • • • • • | 
          
              | Publication | 
              
                | First Author: | Hangen E |  
                | Year: | 2015 |  
                | Journal: | Mol Cell |  
                | Title: | Interaction between AIF and CHCHD4 Regulates Respiratory Chain Biogenesis. |  
                | Volume: | 58 |  
                | Issue: | 6 |  
                | Pages: | 1001-14 |  | • • • • • | 
          
              | Publication | 
              
                | First Author: | Sun Y |  
                | Year: | 2017 |  
                | Journal: | Cell Death Dis |  
                | Title: | Haploinsufficiency in the mitochondrial protein CHCHD4 reduces brain injury in a mouse model of neonatal hypoxia-ischemia. |  
                | Volume: | 8 |  
                | Issue: | 5 |  
                | Pages: | e2781 |  | • • • • • | 
          
              | Publication | 
              
                | First Author: | Hofmann S |  
                | Year: | 2005 |  
                | Journal: | J Mol Biol |  
                | Title: | Functional and mutational characterization of human MIA40 acting during import into the mitochondrial intermembrane space. |  
                | Volume: | 353 |  
                | Issue: | 3 |  
                | Pages: | 517-28 |  | • • • • • | 
          
              | Publication | 
              
                | First Author: | Fischer M |  
                | Year: | 2013 |  
                | Journal: | Mol Biol Cell |  
                | Title: | Protein import and oxidative folding in the mitochondrial intermembrane space of intact mammalian cells. |  
                | Volume: | 24 |  
                | Issue: | 14 |  
                | Pages: | 2160-70 |  | • • • • • | 
          
              | Publication | 
              
                | First Author: | Banci L |  
                | Year: | 2010 |  
                | Journal: | Proc Natl Acad Sci U S A |  
                | Title: | Molecular chaperone function of Mia40 triggers consecutive induced folding steps of the substrate in mitochondrial protein import. |  
                | Volume: | 107 |  
                | Issue: | 47 |  
                | Pages: | 20190-5 |  | • • • • • | 
          
              | Publication | 
              
                | First Author: | Petrungaro C |  
                | Year: | 2015 |  
                | Journal: | Cell Metab |  
                | Title: | The Ca(2+)-Dependent Release of the Mia40-Induced MICU1-MICU2 Dimer from MCU Regulates Mitochondrial Ca(2+) Uptake. |  
                | Volume: | 22 |  
                | Issue: | 4 |  
                | Pages: | 721-33 |  | • • • • • | 
          
              | Protein | 
              
                | Organism: | Mus musculus/domesticus |  
                | Length: | 139  |  
                | Fragment?: | false |  | • • • • • | 
          
              | Protein | 
              
                | Organism: | Mus musculus/domesticus |  
                | Length: | 263  |  
                | Fragment?: | false |  | • • • • • | 
          
              | Protein | 
              
                | Organism: | Mus musculus/domesticus |  
                | Length: | 217  |  
                | Fragment?: | false |  | • • • • • | 
          
              | Publication | 
              
                | First Author: | Modjtahedi N |  
                | Year: | 2015 |  
                | Journal: | Cell Cycle |  
                | Title: | Metabolic epistasis among apoptosis-inducing factor and the mitochondrial import factor CHCHD4. |  
                | Volume: | 14 |  
                | Issue: | 17 |  
                | Pages: | 2743-7 |  | • • • • • | 
          
              | Publication | 
              
                | First Author: | Ma J |  
                | Year: | 2024 |  
                | Journal: | Cell Rep |  
                | Title: | CHCHD4-TRIAP1 regulation of innate immune signaling mediates skeletal muscle adaptation to exercise. |  
                | Volume: | 43 |  
                | Issue: | 1 |  
                | Pages: | 113626 |  | • • • • • | 
          
              | Publication | 
              
                | First Author: | Zhuang J |  
                | Year: | 2013 |  
                | Journal: | Proc Natl Acad Sci U S A |  
                | Title: | Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity. |  
                | Volume: | 110 |  
                | Issue: | 43 |  
                | Pages: | 17356-61 |  | • • • • • | 
          
              | Publication | 
              
                | First Author: | Zhuang J |  
                | Year: | 2016 |  
                | Journal: | J Biol Chem |  
                | Title: | Forkhead Box O3A (FOXO3) and the Mitochondrial Disulfide Relay Carrier (CHCHD4) Regulate p53 Protein Nuclear Activity in Response to Exercise. |  
                | Volume: | 291 |  
                | Issue: | 48 |  
                | Pages: | 24819-24827 |  | • • • • • | 
          
              | Protein Coding Gene | 
              
                | Type: | protein_coding_gene |  
                | Organism: | Mus caroli |  | • • • • • | 
          
              | Protein Coding Gene | 
              
                | Type: | protein_coding_gene |  
                | Organism: | mouse, laboratory |  | • • • • • | 
          
              | Protein Coding Gene | 
              
                | Type: | protein_coding_gene |  
                | Organism: | mouse, laboratory |  | • • • • • | 
          
              | Protein Coding Gene | 
              
                | Type: | protein_coding_gene |  
                | Organism: | mouse, laboratory |  | • • • • • | 
          
              | Protein Coding Gene | 
              
                | Type: | protein_coding_gene |  
                | Organism: | mouse, laboratory |  | • • • • • | 
          
              | Protein Coding Gene | 
              
                | Type: | protein_coding_gene |  
                | Organism: | mouse, laboratory |  | • • • • • | 
          
              | Protein Coding Gene | 
              
                | Type: | protein_coding_gene |  
                | Organism: | mouse, laboratory |  | • • • • • | 
          
              | Protein Coding Gene | 
              
                | Type: | protein_coding_gene |  
                | Organism: | mouse, laboratory |  | • • • • • | 
          
              | Protein Coding Gene | 
              
                | Type: | protein_coding_gene |  
                | Organism: | mouse, laboratory |  | • • • • • | 
          
              | Protein Coding Gene | 
              
                | Type: | protein_coding_gene |  
                | Organism: | mouse, laboratory |  | • • • • • | 
          
              | Protein Coding Gene | 
              
                | Type: | protein_coding_gene |  
                | Organism: | mouse, laboratory |  | • • • • • | 
          
              | Protein Coding Gene | 
              
                | Type: | protein_coding_gene |  
                | Organism: | mouse, laboratory |  | • • • • • | 
          
              | Protein Coding Gene | 
              
                | Type: | protein_coding_gene |  
                | Organism: | mouse, laboratory |  | • • • • • | 
          
              | Protein Coding Gene | 
              
                | Type: | protein_coding_gene |  
                | Organism: | mouse, laboratory |  | • • • • • | 
          
              | Protein Coding Gene | 
              
                | Type: | protein_coding_gene |  
                | Organism: | mouse, laboratory |  | • • • • • | 
          
              | Protein Coding Gene | 
              
                | Type: | protein_coding_gene |  
                | Organism: | mouse, laboratory |  | • • • • • | 
          
              | Protein Coding Gene | 
              
                | Type: | protein_coding_gene |  
                | Organism: | mouse, laboratory |  | • • • • • | 
          
              | Protein Coding Gene | 
              
                | Type: | protein_coding_gene |  
                | Organism: | Mus pahari |  | • • • • • | 
          
              | Protein Coding Gene | 
              
                | Type: | protein_coding_gene |  
                | Organism: | Mus spretus |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Not Specified |  
                | Emaps: | EMAPS:160398 |  
                |  |  
                | Stage: | TS08 |  
                | Assay Id: | MGI:7593892 |  
                | Age: | embryonic day 5.5-6.0 |  
                |  |  
                |  |  
                | Specimen Label: | +/+ |  
                | Detected: | true |  
                | Specimen Num: | 1 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Absent |  
                | Sex: | Not Specified |  
                | Emaps: | EMAPS:160398 |  
                |  |  
                | Stage: | TS08 |  
                | Assay Id: | MGI:7593892 |  
                | Age: | embryonic day 5.5-6.0 |  
                |  |  
                |  |  
                | Specimen Label: | -/- |  
                | Detected: | false |  
                | Specimen Num: | 2 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593906 |  
                | Age: | postnatal day 5 |  
                |  |  
                |  |  
                | Specimen Label: | WT |  
                | Detected: | true |  
                | Specimen Num: | 1 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593906 |  
                | Age: | postnatal day 5 |  
                |  |  
                |  |  
                | Specimen Label: | WT |  
                | Detected: | true |  
                | Specimen Num: | 2 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593906 |  
                | Age: | postnatal day 5 |  
                |  |  
                |  |  
                | Specimen Label: | WT |  
                | Detected: | true |  
                | Specimen Num: | 3 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593906 |  
                | Age: | postnatal day 5 |  
                |  |  
                |  |  
                | Specimen Label: | HQ |  
                | Detected: | true |  
                | Specimen Num: | 4 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593906 |  
                | Age: | postnatal day 5 |  
                |  |  
                |  |  
                | Specimen Label: | HQ |  
                | Detected: | true |  
                | Specimen Num: | 5 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593906 |  
                | Age: | postnatal day 5 |  
                |  |  
                |  |  
                | Specimen Label: | HQ |  
                | Detected: | true |  
                | Specimen Num: | 6 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593906 |  
                | Age: | postnatal day 10 |  
                |  |  
                |  |  
                | Specimen Label: | WT |  
                | Detected: | true |  
                | Specimen Num: | 7 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593906 |  
                | Age: | postnatal day 10 |  
                |  |  
                |  |  
                | Specimen Label: | WT |  
                | Detected: | true |  
                | Specimen Num: | 8 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593906 |  
                | Age: | postnatal day 10 |  
                |  |  
                |  |  
                | Specimen Label: | WT |  
                | Detected: | true |  
                | Specimen Num: | 9 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593906 |  
                | Age: | postnatal day 10 |  
                |  |  
                |  |  
                | Specimen Label: | HQ |  
                | Detected: | true |  
                | Specimen Num: | 10 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593906 |  
                | Age: | postnatal day 10 |  
                |  |  
                |  |  
                | Specimen Label: | HQ |  
                | Detected: | true |  
                | Specimen Num: | 11 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593906 |  
                | Age: | postnatal day 10 |  
                |  |  
                |  |  
                | Specimen Label: | HQ |  
                | Detected: | true |  
                | Specimen Num: | 12 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593906 |  
                | Age: | postnatal day 30 |  
                |  |  
                |  |  
                | Specimen Label: | WT |  
                | Detected: | true |  
                | Specimen Num: | 13 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593906 |  
                | Age: | postnatal day 30 |  
                |  |  
                |  |  
                | Specimen Label: | WT |  
                | Detected: | true |  
                | Specimen Num: | 14 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593906 |  
                | Age: | postnatal day 30 |  
                |  |  
                |  |  
                | Specimen Label: | WT |  
                | Detected: | true |  
                | Specimen Num: | 15 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593906 |  
                | Age: | postnatal day 30 |  
                |  |  
                |  |  
                | Specimen Label: | HQ |  
                | Detected: | true |  
                | Specimen Num: | 16 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593906 |  
                | Age: | postnatal day 30 |  
                |  |  
                |  |  
                | Specimen Label: | HQ |  
                | Detected: | true |  
                | Specimen Num: | 17 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593906 |  
                | Age: | postnatal day 30 |  
                |  |  
                |  |  
                | Specimen Label: | HQ |  
                | Detected: | true |  
                | Specimen Num: | 18 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593906 |  
                | Age: | postnatal day 60 |  
                |  |  
                |  |  
                | Specimen Label: | WT |  
                | Detected: | true |  
                | Specimen Num: | 19 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593906 |  
                | Age: | postnatal day 60 |  
                |  |  
                |  |  
                | Specimen Label: | WT |  
                | Detected: | true |  
                | Specimen Num: | 20 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593906 |  
                | Age: | postnatal day 60 |  
                |  |  
                |  |  
                | Specimen Label: | WT |  
                | Detected: | true |  
                | Specimen Num: | 21 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593906 |  
                | Age: | postnatal day 60 |  
                |  |  
                |  |  
                | Specimen Label: | HQ |  
                | Detected: | true |  
                | Specimen Num: | 22 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593906 |  
                | Age: | postnatal day 60 |  
                |  |  
                |  |  
                | Specimen Label: | HQ |  
                | Detected: | true |  
                | Specimen Num: | 23 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593906 |  
                | Age: | postnatal day 60 |  
                |  |  
                |  |  
                | Specimen Label: | HQ |  
                | Detected: | true |  
                | Specimen Num: | 24 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593940 |  
                | Age: | postnatal day 5 |  
                |  |  
                |  |  
                | Specimen Label: | WT |  
                | Detected: | true |  
                | Specimen Num: | 1 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593940 |  
                | Age: | postnatal day 5 |  
                |  |  
                |  |  
                | Specimen Label: | WT |  
                | Detected: | true |  
                | Specimen Num: | 2 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593940 |  
                | Age: | postnatal day 5 |  
                |  |  
                |  |  
                | Specimen Label: | HQ |  
                | Detected: | true |  
                | Specimen Num: | 3 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593940 |  
                | Age: | postnatal day 5 |  
                |  |  
                |  |  
                | Specimen Label: | HQ |  
                | Detected: | true |  
                | Specimen Num: | 4 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593940 |  
                | Age: | postnatal day 10 |  
                |  |  
                |  |  
                | Specimen Label: | WT |  
                | Detected: | true |  
                | Specimen Num: | 5 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593940 |  
                | Age: | postnatal day 10 |  
                |  |  
                |  |  
                | Specimen Label: | WT |  
                | Detected: | true |  
                | Specimen Num: | 6 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593940 |  
                | Age: | postnatal day 10 |  
                |  |  
                |  |  
                | Specimen Label: | HQ |  
                | Detected: | true |  
                | Specimen Num: | 7 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593940 |  
                | Age: | postnatal day 10 |  
                |  |  
                |  |  
                | Specimen Label: | HQ |  
                | Detected: | true |  
                | Specimen Num: | 8 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593940 |  
                | Age: | postnatal day 30 |  
                |  |  
                |  |  
                | Specimen Label: | WT |  
                | Detected: | true |  
                | Specimen Num: | 9 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593940 |  
                | Age: | postnatal day 30 |  
                |  |  
                |  |  
                | Specimen Label: | WT |  
                | Detected: | true |  
                | Specimen Num: | 10 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593940 |  
                | Age: | postnatal day 30 |  
                |  |  
                |  |  
                | Specimen Label: | HQ |  
                | Detected: | true |  
                | Specimen Num: | 11 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593940 |  
                | Age: | postnatal day 30 |  
                |  |  
                |  |  
                | Specimen Label: | HQ |  
                | Detected: | true |  
                | Specimen Num: | 12 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593940 |  
                | Age: | postnatal day 60 |  
                |  |  
                |  |  
                | Specimen Label: | WT |  
                | Detected: | true |  
                | Specimen Num: | 13 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593940 |  
                | Age: | postnatal day 60 |  
                |  |  
                |  |  
                | Specimen Label: | WT |  
                | Detected: | true |  
                | Specimen Num: | 14 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593940 |  
                | Age: | postnatal day 60 |  
                |  |  
                |  |  
                | Specimen Label: | HQ |  
                | Detected: | true |  
                | Specimen Num: | 15 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593940 |  
                | Age: | postnatal day 60 |  
                |  |  
                |  |  
                | Specimen Label: | HQ |  
                | Detected: | true |  
                | Specimen Num: | 16 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593946 |  
                | Age: | postnatal adult |  
                |  |  
                |  |  
                | Specimen Label: | WT |  
                | Detected: | true |  
                | Specimen Num: | 1 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593946 |  
                | Age: | postnatal adult |  
                |  |  
                |  |  
                | Specimen Label: | WT |  
                | Detected: | true |  
                | Specimen Num: | 2 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593946 |  
                | Age: | postnatal adult |  
                |  |  
                |  |  
                | Specimen Label: | HQ |  
                | Detected: | true |  
                | Specimen Num: | 4 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593946 |  
                | Age: | postnatal adult |  
                |  |  
                |  |  
                | Specimen Label: | HQ |  
                | Detected: | true |  
                | Specimen Num: | 5 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593946 |  
                | Age: | postnatal adult |  
                |  |  
                |  |  
                | Specimen Label: | WT |  
                | Detected: | true |  
                | Specimen Num: | 3 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1689428 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593946 |  
                | Age: | postnatal adult |  
                |  |  
                |  |  
                | Specimen Label: | HQ |  
                | Detected: | true |  
                | Specimen Num: | 6 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1876728 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593946 |  
                | Age: | postnatal adult |  
                |  |  
                |  |  
                | Specimen Label: | WT |  
                | Detected: | true |  
                | Specimen Num: | 7 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1876728 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593946 |  
                | Age: | postnatal adult |  
                |  |  
                |  |  
                | Specimen Label: | WT |  
                | Detected: | true |  
                | Specimen Num: | 8 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1876728 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593946 |  
                | Age: | postnatal adult |  
                |  |  
                |  |  
                | Specimen Label: | WT |  
                | Detected: | true |  
                | Specimen Num: | 9 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1876728 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593946 |  
                | Age: | postnatal adult |  
                |  |  
                |  |  
                | Specimen Label: | HQ |  
                | Detected: | true |  
                | Specimen Num: | 10 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1876728 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593946 |  
                | Age: | postnatal adult |  
                |  |  
                |  |  
                | Specimen Label: | HQ |  
                | Detected: | true |  
                | Specimen Num: | 11 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1876728 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593946 |  
                | Age: | postnatal adult |  
                |  |  
                |  |  
                | Specimen Label: | HQ |  
                | Detected: | true |  
                | Specimen Num: | 12 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1684628 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593946 |  
                | Age: | postnatal adult |  
                |  |  
                |  |  
                | Specimen Label: | WT |  
                | Detected: | true |  
                | Specimen Num: | 13 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1684628 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593946 |  
                | Age: | postnatal adult |  
                |  |  
                |  |  
                | Specimen Label: | WT |  
                | Detected: | true |  
                | Specimen Num: | 14 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1684628 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593946 |  
                | Age: | postnatal adult |  
                |  |  
                |  |  
                | Specimen Label: | WT |  
                | Detected: | true |  
                | Specimen Num: | 15 |  | • • • • • | 
          
              | GXD Expression | 
              
                | Probe: | MGI:7587848 |  
                | Assay Type: | Western blot |  
                | Annotation Date: | 2024-02-16 |  
                | Strength: | Present |  
                | Sex: | Male |  
                | Emaps: | EMAPS:1684628 |  
                |  |  
                | Stage: | TS28 |  
                | Assay Id: | MGI:7593946 |  
                | Age: | postnatal adult |  
                |  |  
                |  |  
                | Specimen Label: | HQ |  
                | Detected: | true |  
                | Specimen Num: | 16 |  | • • • • • |