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Search results 1 to 100 out of 342 for Dhfr

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Type Details Score
Gene
Type: gene
Organism: human
Gene
Type: gene
Organism: dog, domestic
Gene
Type: gene
Organism: cattle
Gene
Type: gene
Organism: chicken
Gene
Type: gene
Organism: zebrafish
Gene
Type: gene
Organism: macaque, rhesus
Gene
Type: gene
Organism: frog, western clawed
Gene
Type: gene
Organism: hamster, Chinese
Gene
Type: gene
Organism: rat
Gene
Type: gene
Organism: chimpanzee
Protein Domain
Type: Family
Description: Dihydrofolate reductase (DHFR) () catalyses the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, an essential step in de novosynthesis both of glycine and of purines and deoxythymidine phosphate (the precursors of DNA synthesis) [], and important also in the conversion of deoxyuridine monophosphate to deoxythymidine monophosphate. Although DHFR is found ubiquitously in prokaryotes and eukaryotes, and is found in all dividing cells, maintaining levels of fully reduced folate coenzymes, the catabolic steps are still not well understood [].Bacterial species possesses distinct DHFR enzymes (based on their pattern of binding diaminoheterocyclic molecules), but mammalian DHFRs are highly similar []. The active site is situated in the N-terminal half of the sequence, which includes a conserved Pro-Trp dipeptide; the tryptophan has been shown []to be involved in the binding of substrate by the enzyme. Its central role in DNA precursor synthesis, coupled with its inhibition by antagonists such as trimethoprim and methotrexate, which are used as anti-bacterial or anti-cancer agents, has made DHFR a target of anticancer chemotherapy. However, resistance has developed against some drugs, as a result of changes in DHFR itself [].
Protein Coding Gene
Type: protein_coding_gene
Organism: mouse, laboratory
Publication
First Author: Snapka RM
Year: 1997
Journal: Cell Prolif
Title: Unbalanced growth in mouse cells with amplified dhfr genes.
Volume: 30
Issue: 10-12
Pages: 385-99
Protein
Organism: Mus musculus/domesticus
Length: 187  
Fragment?: false
Protein
Organism: Mus musculus/domesticus
Length: 187  
Fragment?: false
Publication
First Author: Smith SL
Year: 1979
Journal: J Biol Chem
Title: Porcine liver dihydrofolate reductase. Purification, properties, and amino acid sequence.
Volume: 254
Issue: 22
Pages: 11475-84
Publication
First Author: Bolin JT
Year: 1982
Journal: J Biol Chem
Title: Crystal structures of Escherichia coli and Lactobacillus casei dihydrofolate reductase refined at 1.7 A resolution. I. General features and binding of methotrexate.
Volume: 257
Issue: 22
Pages: 13650-62
Publication
First Author: Cowman AF
Year: 1989
Journal: Mol Cell Biol
Title: Antifolate drug selection results in duplication and rearrangement of chromosome 7 in Plasmodium chabaudi.
Volume: 9
Issue: 11
Pages: 5182-8
Publication
First Author: Oefner C
Year: 1988
Journal: Eur J Biochem
Title: Crystal structure of human dihydrofolate reductase complexed with folate.
Volume: 174
Issue: 2
Pages: 377-85
Publication
First Author: Trimble JJ
Year: 1988
Journal: Science
Title: A gene for dihydrofolate reductase in a herpesvirus.
Volume: 239
Issue: 4844
Pages: 1145-7
Publication
First Author: Linton JP
Year: 1989
Journal: Mol Cell Biol
Title: Dual bidirectional promoters at the mouse dhfr locus: cloning and characterization of two mRNA classes of the divergently transcribed Rep-1 gene.
Volume: 9
Issue: 7
Pages: 3058-72
Publication
First Author: Thoms JA
Year: 2016
Journal: Mol Cell Biol
Title: Arrested Hematopoiesis and Vascular Relaxation Defects in Mice with a Mutation in Dhfr.
Volume: 36
Issue: 8
Pages: 1222-36
Interaction Term
Publication
First Author: Funanage VL
Year: 1984
Journal: Mol Cell Biol
Title: Assignment of the human dihydrofolate reductase gene to the q11----q22 region of chromosome 5.
Volume: 4
Issue: 10
Pages: 2010-6
Publication
First Author: Hughes L
Year: 2015
Journal: FEBS Lett
Title: An active second dihydrofolate reductase enzyme is not a feature of rat and mouse, but they do have activity in their mitochondria.
Volume: 589
Issue: 15
Pages: 1855-62
Publication
First Author: Hanson C
Year: 1990
Journal: Cytogenet Cell Genet
Title: Assignment of two rat dihydrofolate (DHFR) genes to chromosomes 2 and 4.
Volume: 53
Issue: 1
Pages: 23-5
Publication
First Author: Crouse GF
Year: 1982
Journal: J Biol Chem
Title: Structure of amplified normal and variant dihydrofolate reductase genes in mouse sarcoma S180 cells.
Volume: 257
Issue: 13
Pages: 7887-97
Publication
First Author: Means AL
Year: 1992
Journal: Mol Cell Biol
Title: The HIP1 binding site is required for growth regulation of the dihydrofolate reductase gene promoter.
Volume: 12
Issue: 3
Pages: 1054-63
Publication
First Author: Crabtree MJ
Year: 2011
Journal: Free Radic Biol Med
Title: Dihydrofolate reductase protects endothelial nitric oxide synthase from uncoupling in tetrahydrobiopterin deficiency.
Volume: 50
Issue: 11
Pages: 1639-46
Publication  
First Author: Li Q
Year: 2019
Journal: Redox Biol
Title: Knockout of dihydrofolate reductase in mice induces hypertension and abdominal aortic aneurysm via mitochondrial dysfunction.
Volume: 24
Pages: 101185
Publication
First Author: Blakley RL
Year: 1998
Journal: Hum Mutat
Title: In vitro mutations in dihydrofolate reductase that confer resistance to methotrexate: potential for clinical application.
Volume: 11
Issue: 4
Pages: 259-63
Publication
First Author: Schreiner B
Year: 2012
Journal: Mol Biol Cell
Title: Role of the AAA protease Yme1 in folding of proteins in the intermembrane space of mitochondria.
Volume: 23
Issue: 22
Pages: 4335-46
Publication
First Author: Killary AM
Year: 1986
Journal: Somat Cell Mol Genet
Title: Assignment of genes encoding dihydrofolate reductase and hexosaminidase B to Mus musculus chromosome 13.
Volume: 12
Issue: 6
Pages: 641-8
Publication
First Author: Crouse GF
Year: 1983
Journal: Mol Cell Biol
Title: Expression and amplification of engineered mouse dihydrofolate reductase minigenes.
Volume: 3
Issue: 2
Pages: 257-66
Publication
First Author: Chen MJ
Year: 1984
Journal: J Biol Chem
Title: The functional human dihydrofolate reductase gene.
Volume: 259
Issue: 6
Pages: 3933-43
Publication
First Author: James RI
Year: 1997
Journal: Exp Hematol
Title: Transgenic mice expressing the tyr22 variant of murine DHFR: protection of transgenic marrow transplant recipients from lethal doses of methotrexate.
Volume: 25
Issue: 12
Pages: 1286-95
Publication
First Author: Cai Z
Year: 2015
Journal: Arterioscler Thromb Vasc Biol
Title: Endothelial Nitric Oxide Synthase-Derived Nitric Oxide Prevents Dihydrofolate Reductase Degradation via Promoting S-Nitrosylation.
Volume: 35
Issue: 11
Pages: 2366-73
Publication
First Author: Crabtree MJ
Year: 2009
Journal: J Biol Chem
Title: Critical role for tetrahydrobiopterin recycling by dihydrofolate reductase in regulation of endothelial nitric-oxide synthase coupling: relative importance of the de novo biopterin synthesis versus salvage pathways.
Volume: 284
Issue: 41
Pages: 28128-36
Publication
First Author: Kuzumaki T
Year: 1996
Journal: Biochim Biophys Acta
Title: Cell adhesion to substratum and activation of tyrosine kinases are essentially required for G1/S phase transition in BALB/c 3T3 fibroblasts.
Volume: 1310
Issue: 2
Pages: 185-92
Publication
First Author: Fry CJ
Year: 1997
Journal: Mol Cell Biol
Title: Position-dependent transcriptional regulation of the murine dihydrofolate reductase promoter by the E2F transactivation domain.
Volume: 17
Issue: 4
Pages: 1966-76
Publication
First Author: Killary AM
Year: 1986
Journal: Am J Hum Genet
Title: Assignment of the genes encoding dihydrofolate reductase and hexosaminidase B to mouse Chromosome 13
Volume: 39
Issue: Suppl
Pages: A159 (Abstr.)
Publication
First Author: Smith ML
Year: 1990
Journal: Mol Cell Biol
Title: Analysis of the mouse Dhfr/Rep-3 major promoter region by using linker-scanning and internal deletion mutations and DNase I footprinting.
Volume: 10
Issue: 11
Pages: 6003-12
Publication  
First Author: Saha S
Year: 2023
Journal: Development
Title: Dihydrofolate reductase activity controls neurogenic transitions in the developing neocortex.
Volume: 150
Issue: 20
Publication
First Author: Weng Q
Year: 2017
Journal: Sci Rep
Title: Folate Metabolism Regulates Oligodendrocyte Survival and Differentiation by Modulating AMPKα Activity.
Volume: 7
Issue: 1
Pages: 1705
Publication
First Author: Beecham EJ
Year: 1997
Journal: Carcinogenesis
Title: Decoupling of DNA excision repair and RNA transcription in translocation breaksite regions of plasmacytoma-susceptible BALB/cAnPt mice.
Volume: 18
Issue: 4
Pages: 687-94
Publication
First Author: Newton G
Year: 1999
Journal: Mamm Genome
Title: The thrombospondin-4 gene.
Volume: 10
Issue: 10
Pages: 1010-6
Publication
First Author: Cao Y
Year: 2019
Journal: Mol Cell Biochem
Title: Endothelial retinoblastoma protein reduces abdominal aortic aneurysm development via promoting DHFR/NO pathway-mediated vasoprotection.
Volume: 460
Issue: 1-2
Pages: 29-36
Protein Coding Gene
Type: protein_coding_gene
Organism: Mus caroli
Protein Coding Gene
Type: protein_coding_gene
Organism: mouse, laboratory
Protein Coding Gene
Type: protein_coding_gene
Organism: mouse, laboratory
Protein Coding Gene
Type: protein_coding_gene
Organism: mouse, laboratory
Protein Coding Gene
Type: protein_coding_gene
Organism: mouse, laboratory
Protein Coding Gene
Type: protein_coding_gene
Organism: mouse, laboratory
Protein Coding Gene
Type: protein_coding_gene
Organism: mouse, laboratory
Protein Coding Gene
Type: protein_coding_gene
Organism: mouse, laboratory
Protein Coding Gene
Type: protein_coding_gene
Organism: mouse, laboratory
Protein Coding Gene
Type: protein_coding_gene
Organism: mouse, laboratory
Protein Coding Gene
Type: protein_coding_gene
Organism: mouse, laboratory
Protein Coding Gene
Type: protein_coding_gene
Organism: mouse, laboratory
Protein Coding Gene
Type: protein_coding_gene
Organism: mouse, laboratory
Protein Coding Gene
Type: protein_coding_gene
Organism: mouse, laboratory
Protein Coding Gene
Type: protein_coding_gene
Organism: mouse, laboratory
Protein Coding Gene
Type: protein_coding_gene
Organism: mouse, laboratory
Protein Coding Gene
Type: protein_coding_gene
Organism: mouse, laboratory
Protein Coding Gene
Type: protein_coding_gene
Organism: Mus pahari
Protein Coding Gene
Type: protein_coding_gene
Organism: Mus spretus
Publication
First Author: Simonsen CC
Year: 1983
Journal: Proc Natl Acad Sci U S A
Title: Isolation and expression of an altered mouse dihydrofolate reductase cDNA.
Volume: 80
Issue: 9
Pages: 2495-9
Publication
First Author: Chang AC
Year: 1978
Journal: Nature
Title: Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase.
Volume: 275
Issue: 5681
Pages: 617-24
Publication
First Author: Tauro GP
Year: 1976
Journal: N Engl J Med
Title: Dihydrofolate reductase deficiency causing megaloblastic anemia in two families.
Volume: 294
Issue: 9
Pages: 466-70
Publication
First Author: Alt FW
Year: 1978
Journal: J Biol Chem
Title: Selective multiplication of dihydrofolate reductase genes in methotrexate-resistant variants of cultured murine cells.
Volume: 253
Issue: 5
Pages: 1357-70
Publication
First Author: Schimke RT
Year: 1978
Journal: Science
Title: Gene amplification and drug resistance in cultured murine cells.
Volume: 202
Issue: 4372
Pages: 1051-5
Publication
First Author: Lee F
Year: 1981
Journal: Nature
Title: Glucocorticoids regulate expression of dihydrofolate reductase cDNA in mouse mammary tumour virus chimaeric plasmids.
Volume: 294
Issue: 5838
Pages: 228-32
Publication
First Author: Xu HP
Year: 1996
Journal: Mamm Genome
Title: New polymorphic markers in the vicinity of the pearl locus on mouse chromosome 13.
Volume: 7
Issue: 1
Pages: 16-9
Publication  
First Author: HAKALA MT
Year: 1961
Journal: J Biol Chem
Title: Relation of folic acid reductase to amethopterin resistance in cultured mammalian cells.
Volume: 236
Pages: 952-8
GXD Expression        
Probe: MGI:5466732
Assay Type: RNase protection
Annotation Date: 2013-03-01
Strength: Not Specified
Sex: Not Specified
Emaps: EMAPS:1684628
Stage: TS28
Assay Id: MGI:5466745
Age: postnatal adult
Specimen Label: liver
Specimen Num: 1
GXD Expression      
Probe: MGI:5466732
Assay Type: RNase protection
Annotation Date: 2013-03-01
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1876728
Stage: TS28
Assay Id: MGI:5466745
Age: postnatal adult
Specimen Label: spleen
Detected: true
Specimen Num: 2
GXD Expression      
Probe: MGI:5466732
Assay Type: RNase protection
Annotation Date: 2013-03-01
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1737328
Stage: TS28
Assay Id: MGI:5466745
Age: postnatal adult
Specimen Label: kidney
Detected: true
Specimen Num: 3
GXD Expression        
Probe: MGI:5466732
Assay Type: RNase protection
Annotation Date: 2013-03-01
Strength: Not Specified
Sex: Not Specified
Emaps: EMAPS:1778728
Stage: TS28
Assay Id: MGI:5466745
Age: postnatal adult
Specimen Label: cerebellum
Specimen Num: 4
GXD Expression        
Probe: MGI:5466732
Assay Type: RNase protection
Annotation Date: 2013-03-01
Strength: Not Specified
Sex: Not Specified
Emaps: EMAPS:1691028
Stage: TS28
Assay Id: MGI:5466745
Age: postnatal adult
Specimen Label: cerebrum
Specimen Num: 5
GXD Expression      
Probe: MGI:5466732
Assay Type: RNase protection
Annotation Date: 2013-03-01
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1876828
Stage: TS28
Assay Id: MGI:5466745
Age: postnatal adult
Specimen Label: thymus
Detected: true
Specimen Num: 6
GXD Expression        
Probe: MGI:5466732
Assay Type: RNase protection
Annotation Date: 2013-03-01
Strength: Not Specified
Sex: Not Specified
Emaps: EMAPS:3263428
Stage: TS28
Assay Id: MGI:5466745
Age: postnatal adult
Specimen Label: thigh muscle
Specimen Num: 7
GXD Expression      
Probe: MGI:5466732
Assay Type: RNase protection
Annotation Date: 2013-03-01
Strength: Present
Sex: Not Specified
Emaps: EMAPS:3185820
Stage: TS20
Assay Id: MGI:5466755
Age: embryonic day 12.5
Specimen Label: FACE
Detected: true
Specimen Num: 2
GXD Expression      
Probe: MGI:5466732
Assay Type: RNase protection
Annotation Date: 2013-03-01
Strength: Present
Sex: Not Specified
Emaps: EMAPS:3185720
Stage: TS20
Assay Id: MGI:5466755
Age: embryonic day 12.5
Specimen Label: BODY
Detected: true
Specimen Num: 3
GXD Expression      
Probe: MGI:5466732
Assay Type: RNase protection
Annotation Date: 2013-03-01
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1689521
Stage: TS21
Assay Id: MGI:5466755
Age: embryonic day 13.5
Specimen Label: Forebrain
Detected: true
Specimen Num: 4
GXD Expression        
Probe: MGI:5466732
Assay Type: RNase protection
Annotation Date: 2013-03-01
Strength: Not Specified
Sex: Not Specified
Emaps: EMAPS:1691621
Stage: TS21
Assay Id: MGI:5466755
Age: embryonic day 13.5
Specimen Label: Hindbrain
Specimen Num: 5
GXD Expression        
Probe: MGI:5466732
Assay Type: RNase protection
Annotation Date: 2013-03-01
Strength: Not Specified
Sex: Not Specified
Emaps: EMAPS:1757721
Stage: TS21
Assay Id: MGI:5466755
Age: embryonic day 13.5
Specimen Label: Spinal cord
Specimen Num: 6
GXD Expression      
Probe: MGI:5466732
Assay Type: RNase protection
Annotation Date: 2013-03-01
Strength: Present
Sex: Not Specified
Emaps: EMAPS:3185821
Stage: TS21
Assay Id: MGI:5466755
Age: embryonic day 13.5
Specimen Label: Face
Detected: true
Specimen Num: 7
GXD Expression      
Probe: MGI:5466732
Assay Type: RNase protection
Annotation Date: 2013-03-01
Strength: Absent
Sex: Not Specified
Emaps: EMAPS:1684621
Stage: TS21
Assay Id: MGI:5466755
Age: embryonic day 13.5
Specimen Label: Liver/gut
Detected: false
Specimen Num: 8
GXD Expression      
Probe: MGI:5466732
Assay Type: RNase protection
Annotation Date: 2013-03-01
Strength: Absent
Sex: Not Specified
Emaps: EMAPS:1624721
Stage: TS21
Assay Id: MGI:5466755
Age: embryonic day 13.5
Specimen Label: Liver/gut
Detected: false
Specimen Num: 8
GXD Expression      
Probe: MGI:5466732
Assay Type: RNase protection
Annotation Date: 2013-03-01
Strength: Present
Sex: Not Specified
Emaps: EMAPS:3185721
Stage: TS21
Assay Id: MGI:5466755
Age: embryonic day 13.5
Specimen Label: Body
Detected: true
Specimen Num: 9
GXD Expression      
Probe: MGI:5466732
Assay Type: RNase protection
Annotation Date: 2013-03-01
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1672821
Stage: TS21
Assay Id: MGI:5466755
Age: embryonic day 13.5
Specimen Label: Heart/lungs
Detected: true
Specimen Num: 10
GXD Expression      
Probe: MGI:5466732
Assay Type: RNase protection
Annotation Date: 2013-03-01
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1610521
Stage: TS21
Assay Id: MGI:5466755
Age: embryonic day 13.5
Specimen Label: Heart/lungs
Detected: true
Specimen Num: 10
GXD Expression      
Probe: MGI:5466732
Assay Type: RNase protection
Annotation Date: 2013-03-01
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1640521
Stage: TS21
Assay Id: MGI:5466755
Age: embryonic day 13.5
Specimen Label: Limbs
Detected: true
Specimen Num: 11
GXD Expression      
Probe: MGI:5466732
Assay Type: RNase protection
Annotation Date: 2013-03-01
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1608521
Stage: TS21
Assay Id: MGI:5466755
Age: embryonic day 13.5
Specimen Label: Yolk sac
Detected: true
Specimen Num: 12
GXD Expression      
Probe: MGI:6196092
Assay Type: RT-PCR
Annotation Date: 2018-08-30
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1684621
Stage: TS21
Assay Id: MGI:6196094
Age: embryonic day 13.5
Specimen Label: +/+
Detected: true
Specimen Num: 1
GXD Expression      
Probe: MGI:6196092
Assay Type: RT-PCR
Annotation Date: 2018-08-30
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1684621
Stage: TS21
Assay Id: MGI:6196094
Age: embryonic day 13.5
Specimen Label: +/Ora
Detected: true
Specimen Num: 2
GXD Expression      
Probe: MGI:6196092
Assay Type: RT-PCR
Annotation Date: 2018-08-30
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1684621
Stage: TS21
Assay Id: MGI:6196094
Age: embryonic day 13.5
Specimen Label: Ora/Ora
Detected: true
Specimen Num: 3
GXD Expression      
Probe: MGI:6196092
Assay Type: RT-PCR
Annotation Date: 2018-08-30
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1684628
Stage: TS28
Assay Id: MGI:6196095
Age: postnatal adult
Specimen Label: +/+
Detected: true
Specimen Num: 1
GXD Expression      
Probe: MGI:6196092
Assay Type: RT-PCR
Annotation Date: 2018-08-30
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1684628
Stage: TS28
Assay Id: MGI:6196095
Age: postnatal adult
Specimen Label: +/Ora
Detected: true
Specimen Num: 2
GXD Expression      
Probe: MGI:6196091
Assay Type: Western blot
Annotation Date: 2018-08-30
Strength: Present
Sex: Not Specified
Emaps: EMAPS:1684621
Stage: TS21
Assay Id: MGI:6196096
Age: embryonic day 13.5
Specimen Label: +/+
Detected: true
Specimen Num: 1