| Primary Identifier | MGI:4849370 | Allele Type | Targeted |
| Attribute String | Inducible, Inserted expressed sequence, RMCE-ready | Gene | Col1a1 |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
| Induced With | doxycycline/tetracycline | Is Recombinase | false |
| Is Wild Type | false |
| description | The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm13(neo/hygro*))Jae. This allele was generated with Col1a1tm13(neo/hygro*)Jae. |
| molecularNote | RMCE on KH2 cells inserted a cassette that contains a splice acceptor, 2 polyadenylation sequence, tetracycline operator, flag-tagged mouse cDNA, and an SV40 polyadenylation signal into the 3'UTR. Leaky ectopic expression in the absence of doxycycline induction was confirmed by quantitative RT-PCR and immunohistochemistry. |
