| First Author | Vestal DJ | Year | 1998 |
| Journal | J Interferon Cytokine Res | Volume | 18 |
| Issue | 11 | Pages | 977-85 |
| PubMed ID | 9858320 | Mgi Jnum | J:51786 |
| Mgi Id | MGI:1326859 | Doi | 10.1089/jir.1998.18.977 |
| Citation | Vestal DJ, et al. (1998) Murine GBP-2: a new IFN-gamma-induced member of the GBP family of GTPases isolated from macrophages. J Interferon Cytokine Res 18(11):977-85 |
| abstractText | We have cloned a new member of the interferon (IFN)- induced guanylate-binding protein (GBP) family of GTPases, murine GBP-2 (mGBP-2), from bone marrow-derived macrophages, mGBP-2 is located on murine chromosome 3, where it is linked to mGBP-1, With the identification of mGBP-2 there are now two human and two murine GBPs, Like other GBPs, mGBP-2 RNA and protein are induced by IFN- gamma, In addition, mGBP-2 shares with the other GBPs important structural features that distinguish this family from other GTPases, First, mGBP-2 contains only two of the three consensus sequences for nucleotide binding found within the classic GTP binding regions of other GTPases, A second amino acid motif found in mGBP-2 is a potential C- terminal site for isoprenoid modification, called a CaaX sequence. MGBP-2 is prenylated, as detected by [H- 3]mevalonate incorporation, when expressed in COS cells and preferentially incorporates the C-20 isoprenoid geranylgeraniol, Surprisingly, despite having a functional CaaX sequence, mGBP-2 is primarily cytosolic, GBP proteins are very abundant in IFN-exposed cells, but little is known about their function. MGBP-2 is expressed by IFN- gamma-treated cells from C57Bl/6 mice, whereas mGBP-1 is not. Thus, the identification of mGBP-2 makes possible the study of GBP function in the absence of a second family member. |
