| First Author | Higuchi Y | Year | 2017 |
| Journal | Sci Rep | Volume | 7 |
| Issue | 1 | Pages | 7028 |
| PubMed ID | 28765635 | Mgi Jnum | J:256520 |
| Mgi Id | MGI:6108603 | Doi | 10.1038/s41598-017-07376-5 |
| Citation | Higuchi Y, et al. (2017) Conditional knockdown of hyaluronidase 2 in articular cartilage stimulates osteoarthritic progression in a mice model. Sci Rep 7(1):7028 |
| abstractText | The catabolism of hyaluronan in articular cartilage remains unclear. The aims of this study were to investigate the effects of hyaluronidase 2 (Hyal2) knockdown in articular cartilage on the development of osteoarthritis (OA) using genetic manipulated mice. Destabilization of the medial meniscus (DMM) model of Col2a promoter specific conditional Hyal2 knockout (Hyal (-/-) ) mice was established and examined. Age related and DMM induced alterations of articular cartilage of knee joint were evaluated with modified Mankin score and immunohistochemical staining of MMP-13, ADAMTS-5, KIAA11199, and biotinylated- hyaluronan binding protein staining in addition to histomorphometrical analyses. Effects of Hyal2 suppression were also analyzed using explant culture of an IL-1alpha induced articular cartilage degradation model. The amount and size of hyaluronan in articular cartilage were higher in Hyal2 (-/-) mice. Hyal2 (-/-) mice exhibited aggravated cartilage degradation in age-related and DMM induced mice. MMP-13 and ADAMTS-5 positive chondrocytes were significantly higher in Hyal2 (-/-) mice. Articular cartilage was more degraded in explant cultures obtained from Hyal2 (-/-) mice. Knockdown of Hyal2 in articular cartilage induced OA development and progression possibly mediated by an imbalance of HA metabolism. This suggests that Hyal2 knockdown exhibits mucopolysaccharidosis-like OA change in articular cartilage similar to Hyal1 knockdown. |
