| First Author | Gaucher D | Year | 2003 |
| Journal | Gene | Volume | 307 |
| Pages | 159-66 | PubMed ID | 12706898 |
| Mgi Jnum | J:83208 | Mgi Id | MGI:2658741 |
| Doi | 10.1016/s0378-1119(03)00457-8 | Citation | Gaucher D, et al. (2003) Gerbil interleukin-18 and caspase-1: cloning, expression and characterization. Gene 307:159-66 |
| abstractText | We are reporting the molecular cloning of gerbil interleukin-18 (IL-18) and caspase-1. The cDNAs encoding the molecules were cloned by cross-species reverse transcriptase-polymerase chain reaction (RT-PCR) and 5'/3' rapid amplification of cDNA ends (RACE). COS-7 cells transfected with plasmids encoding pro-IL-18 and caspase-1 precursor expressed the two proteins intracellularly. When the cells were lysed in the presence of dithiothreitol, caspase-1 precursor became active and converted pro-IL-18 into mature IL-18. A partially purified preparation of gerbil mature IL-18 was bioactive, as it stimulated the proliferation of gerbil spleen cells in a dose-dependent manner. |
