| First Author | Hofmann M | Year | 2004 |
| Journal | Eur J Immunol | Volume | 34 |
| Issue | 7 | Pages | 1798-806 |
| PubMed ID | 15214028 | Mgi Jnum | J:90766 |
| Mgi Id | MGI:3044554 | Doi | 10.1002/eji.200425088 |
| Citation | Hofmann M, et al. (2004) T cell avidity determines the level of CTL activation. Eur J Immunol 34(7):1798-806 |
| abstractText | To investigate the influence of avidity on T cell activation in vitro and in vivo, we analyzed T cells from St40 and St42 mice, which express the same transgenic TCR specific for an E1a-derived epitope of adenovirus type 5 with different expression levels and therefore different avidities. Splenocytes from both strains showed comparable cytolytic activities and required identical peptide concentrations for efficient target cell lysis and up-regulation of activation markers. However, the kinetics of CD25 up-regulation were strikingly different: whereas the majority of the high-avidity St42 T cells up-regulated the IL-2Ralpha chain within a few hours, low-avidity St40 T cells expressed only 50% of the CD25 of high-avidity T cells after 2 days. In addition, low-avidity T cells proliferated poorly and displayed impaired secretion of IL-2 and IFN-gamma. Similar results were seen with high-avidity St42 T cells stimulated with a partial agonistic peptide. Upon adoptive transfer and subsequent immunization with adenovirus, both high- and low-avidity T cells expanded, but St40 T cells were undetectable 10 days after immunization. Our model system now allows analysis of whether T cells with identical specificities but different avidities influence each other during activation and homeostatic proliferation. |
