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Publication : Cloning and characterization of the murine homologue of the rat/human MRC OX-2 gene.

First Author  Chen Z Year  1997
Journal  Biochim Biophys Acta Volume  1362
Issue  1 Pages  6-10
PubMed ID  9434094 Mgi Jnum  J:44890
Mgi Id  MGI:1101436 Doi  10.1016/s0925-4439(97)00058-6
Citation  Chen Z, et al. (1997) Cloning and characterization of the murine homologue of the rat/human MRC OX-2 gene. Biochim Biophys Acta 1362(1):6-10
abstractText  A 350 bp amplicon, obtained by PCR-select subtractive hybridization from RNA derived from mesenteric lymph nodes (MLN) cells from mice pre-immunized with allogeneic lymphocytes 36hrs prior to receiving donor-specific skin grafts, and showing > 98% homology with a published sequence for the rat MRC OX-2 gene, was used as a hybridization probe to screen a cDNA library constructed from adult mouse MLN treated in the same fashion. Several clones were identified which, on DNA sequence analysis, predicted a 218 amino acid protein showing significant homology with the rat and human MRC OX-2 gene product.
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