First Author | Chen Z | Year | 1997 |
Journal | Biochim Biophys Acta | Volume | 1362 |
Issue | 1 | Pages | 6-10 |
PubMed ID | 9434094 | Mgi Jnum | J:44890 |
Mgi Id | MGI:1101436 | Doi | 10.1016/s0925-4439(97)00058-6 |
Citation | Chen Z, et al. (1997) Cloning and characterization of the murine homologue of the rat/human MRC OX-2 gene. Biochim Biophys Acta 1362(1):6-10 |
abstractText | A 350 bp amplicon, obtained by PCR-select subtractive hybridization from RNA derived from mesenteric lymph nodes (MLN) cells from mice pre-immunized with allogeneic lymphocytes 36hrs prior to receiving donor-specific skin grafts, and showing > 98% homology with a published sequence for the rat MRC OX-2 gene, was used as a hybridization probe to screen a cDNA library constructed from adult mouse MLN treated in the same fashion. Several clones were identified which, on DNA sequence analysis, predicted a 218 amino acid protein showing significant homology with the rat and human MRC OX-2 gene product. |