Primary Identifier | MGI:7537121 | Allele Type | Targeted |
Attribute String | Conditional ready | Gene | Ank2 |
Transmission | Germline | Strain of Origin | C57BL/6J |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The targeting vector is designed to contain (from 5'; to 3') an FRT site, an En2 splice acceptor, an internal ribosome entry site (IRES) fused to an EGFP sequence with a polyadenylation signal, and a floxed cassette containing a neo and a second FRT site all upstream of exon 4. A third loxP was placed downstream of exon 4. The frt-flanked neomycin cassette was removed in the germline via FLP-mediated recombination. |