| First Author | Konno R | Year | 1996 |
| Journal | Amino Acids | Volume | 10 |
| Issue | 2 | Pages | 133-43 |
| PubMed ID | 24178475 | Mgi Jnum | J:33329 |
| Mgi Id | MGI:80810 | Doi | 10.1007/BF00806586 |
| Citation | Konno R, et al. (1996) Mouse D-amino-acid oxidase gene: Restriction fragment length polymorphism among mouse strains. Amino Acids 10(2):133-143 |
| abstractText | Genomic DNA was extracted from mice of 15 strains (A/J, AKR, BALB/c, C3H/He, C57BL/6, CBA/J, CD-1, CF#1, DBA/2, ddY/DAO(+), ddY/DAO(-), ICR, NC, NZB and NZW) for the examination of the difference in the structure of the D- amino-acid oxidase gene among the mouse strains. The DNAs were digested with restriction endonucleases and analyzed by Southern hybridization using D-amino-acid oxidase cDNA as a probe. The 15 strains showed the same hybridization patterns in the EcoRV, BamHI or BglII digestion. In the EcoRI digestion, the DBA/2 strain showed a different hybridization pattern from the other 14 strains. In the PvuII and XbaI digestion, C3H/He, CBA/J, ddY/DAO(+) and NC strains were different from the other 11 strains. In the PstI and HindIII digestion, restriction fragment length polymorphisms were observed, and the 15 strains were classified into four groups according to their hybridization patterns. These results indicate that the 15 strains of mice carry a structurally similar D-amino-acid oxidase gene, but there is a variation in its inside sequence among the groups of the strains. |
