| First Author | Gumley TP | Year | 1992 |
| Journal | J Immunol | Volume | 149 |
| Issue | 8 | Pages | 2615-8 |
| PubMed ID | 1401899 | Mgi Jnum | J:2870 |
| Mgi Id | MGI:51390 | Doi | 10.4049/jimmunol.149.8.2615 |
| Citation | Gumley TP, et al. (1992) Isolation and characterization of cDNA clones for the mouse thymocyte B cell antigen (ThB). J Immunol 149(8):2615-8 |
| abstractText | The Thb locus is responsible for the expression of 15-kDa phosphatidyl inositol anchored molecules (ThB) on murine thymocytes and B cells. Thb expression as detected with mAb is polymorphic on B cells with two alleles, Thbh and Thb1 responsible for the high and low expression of ThB on B cells. The regulatory locus for Thb expression had been mapped with the Ly-6 cluster of genes to Chr 15. In our study we used expression cloning in COS cells to isolate cDNA clones that code for ThB after transfection; the cDNA products react with anti- ThB antibodies, but not with Ly-6A.2, -6B.2, -6C.2, or -6D.2 antibodies. One of these clones, pThB-A contains insert of 702 bases which was sequenced. The translated amino acid sequence has 11 cysteine residues, and together with the absence of potential N-linked glycosylation sites is similar to the structure of the Ly-6 molecules. The nucleotide and amino acid sequences of ThB cDNA were compared to those of Ly-6 genes and the Ly-6 related human CD59 and show clear homology. Finally using interspecies crosses, the structural Thb gene has been mapped to Chr 15; thus both structural and regulatory genes map to a similar site. The genetic map location near Ly-6 and the sequence similarity suggest that Thb and Ly-6 may have been derived from the same progenitor by gene duplication. |
