| First Author | Serre K | Year | 2011 |
| Journal | Eur J Immunol | Volume | 41 |
| Issue | 6 | Pages | 1573-82 |
| PubMed ID | 21469117 | Mgi Jnum | J:176487 |
| Mgi Id | MGI:5291904 | Doi | 10.1002/eji.201041126 |
| Citation | Serre K, et al. (2011) Selective effects of NF-kappaB1 deficiency in CD4 T cells on Th2 and TFh induction by alum-precipitated protein vaccines. Eur J Immunol 41(6):1573-82 |
| abstractText | NF-kappaB1-dependent signaling directs the development of CD4(+) Th2 cells during allergic airway inflammation and protective responses to helminth infection. Here, we show that IL-4 and IL-13 production is NF-kappaB1-dependent in mouse OVA-specific CD4(+) (OTII) T cells responding to alum-precipitated OVA (alumOVA) immunization. More surprisingly, we found that NF-kappaB1 deficiency in OTII cells also selectively impairs their CXCR5 induction by alumOVA without affecting upregulation of BCL6, IL-21, OX40 and CXCR4 mRNA and PD-1 protein. This results in functional impairment of follicular helper T cells. Thus, fewer germinal center B cells develop in LN responses to alumOVA in T-cell-deficient mice reconstituted with NF-kappaB1(-/-) OTII cells as opposed to NF-kappaB1(+/+) OTII cells, while plasma cell numbers are comparable. Unlike CXCR5 induction in CD4(+) T cells, NF-kappaB1-deficient recirculating follicular B cells are shown to express normal levels of CXCR5. The selective effects of NF-kappaB1-deficiency on Th2 and follicular helper T cell induction do not appear to be due to altered expression of the Th2-associated transcription factors - GATA-3, c-Maf and Ikaros. Altogether, these results suggest that NF-kappaB1 regulates the expression of CXCR5 on CD4(+) T cells primed in vivo, and thus selectively controls the T-cell-dependent germinal center component of B-cell response to alumOVA. |
