| First Author | Chao W | Year | 2002 |
| Journal | Science | Volume | 295 |
| Issue | 5553 | Pages | 345-7 |
| PubMed ID | 11743158 | Mgi Jnum | J:73834 |
| Mgi Id | MGI:2156936 | Doi | 10.1126/science.1065982 |
| Citation | Chao W, et al. (2002) CTCF, a candidate trans-acting factor for X-inactivation choice. Science 295(5553):345-7 |
| abstractText | In mammals, X-inactivation silences one of two female X chromosomes. Silencing depends on the noncoding gene, Xist (inactive X-specific transcript), and is blocked by the antisense gene, Tsix. Deleting the choice/imprinting center in Tsix affects X-chromosome selection. Here, we identify the insulator and transcription factor, CTCF, as a candidate trans-acting factor for X-chromosome selection. The choice/imprinting center contains tandem CTCF binding sites that function in an enhancer-blocking assay. In vitro binding is reduced by CpG methylation and abolished by including non-CpG methylation. We postulate that Tsix and CTCF together establish a regulatable epigenetic switch for X-inactivation. |
