| First Author | Tanino T | Year | 1992 |
| Journal | Cancer Immunol Immunother | Volume | 35 |
| Issue | 4 | Pages | 230-6 |
| PubMed ID | 1380893 | Mgi Jnum | J:2180 |
| Mgi Id | MGI:50704 | Doi | 10.1007/BF01789328 |
| Citation | Tanino T, et al. (1992) Detection of allogeneic Qa/TL and Ly specificities on murine tumor cells with IgD in tumor-regressor serum. Cancer Immunol Immunother 35(4):230-6 |
| abstractText | Serum from C3H/He mice, which show regression of MM2 tumor cells after transplantation and removal (regressor serum, RS) contains non-gammaglobulin components that cross-react with various tumor cells of mice [22, 23]. In addition to tumor cells, various allogeneic lymphocytes are also susceptible to an RS-dependent lymphocyte-mediated cytotoxic reaction. To identify tumor cell surface antigens that cause the cross-reactive host response, the serum components were analyzed by absorption of RS with allogeneic lymphocytes. RS components were found to recognize allogeneic lymphocyte antigens including Qa-2 and Ly6.2. Specificity for the Qa-2 antigen was further tested using Qa-2-congenic mice. The expression of Qa-2 antigen was detected on the surfaces of MM2 and other tumor cells derived from H-2k mice (seven among nine cell lines tested) by a membrane immunofluorescence method using a Qa-2-specific mAb. Physical characteristics of the Qa-2-specific component in RS were determined and found to differ from those of regular IgGs but to be similar to those of IgDs. Using an enzyme-linked immunosorbent assay with an IgD-specific mAb and Qa-2-lacZ fusion protein, the existence of IgD in RS with specificity for Qa-2 was confirmed. These results suggest that the RS component with Qa-2 specificity is an IgD, the specificity and physiological role of which are unknown. |
