| First Author | Ono Y | Year | 2019 |
| Journal | Sci Rep | Volume | 9 |
| Issue | 1 | Pages | 4289 |
| PubMed ID | 30862855 | Mgi Jnum | J:304586 |
| Mgi Id | MGI:6695685 | Doi | 10.1038/s41598-019-40557-y |
| Citation | Ono Y, et al. (2019) Expression of plasma membrane calcium ATPases confers Ca(2+)/H(+) exchange in rodent synaptic vesicles. Sci Rep 9(1):4289 |
| abstractText | Ca(2+) transport into synaptic vesicles (SVs) at the presynaptic terminals has been proposed to be an important process for regulating presynaptic [Ca(2+)] during stimulation as well as at rest. However, the molecular identity of the transport system remains elusive. Previous studies have demonstrated that isolated SVs exhibit two distinct Ca(2+) transport systems depending on extra-vesicular (cytosolic) pH; one is mediated by a high affinity Ca(2+) transporter which is active at neutral pH and the other is mediated by a low affinity Ca(2+)/H(+) antiporter which is maximally active at alkaline pH of 8.5. In addition, synaptic vesicle glycoprotein 2 s (SV2s), a major SV component, have been proposed to contribute to Ca(2+) clearance from the presynaptic cytoplasm. Here, we show that at physiological pH, the plasma membrane Ca(2+) ATPases (PMCAs) are responsible for both the Ca(2+)/H(+) exchange activity and Ca(2+) uptake into SVs. The Ca(2+)/H(+) exchange activity monitored by acidification assay exhibited high affinity for Ca(2+) (Km ~ 400 nM) and characteristic divalent cation selectivity for the PMCAs. Both activities were remarkably reduced by PMCA blockers, but not by a blocker of the ATPase that transfers Ca(2+) from the cytosol to the lumen of sarcoplasmic endoplasmic reticulum (SERCA) at physiological pH. Furthermore, we rule out the contribution of SV2s, putative Ca(2+) transporters on SVs, since both Ca(2+)/H(+) exchange activity and Ca(2+) transport were unaffected in isolated vesicles derived from SV2-deficient brains. Finally, using a PMCA1-pHluorin construct that enabled us to monitor cellular distribution and recycling properties in living neurons, we demonstrated that PMCA1-pHluorin localized to intracellular acidic compartments and recycled at presynaptic terminals in an activity-dependent manner. Collectively, our results imply that vesicular PMCAs may play pivotal roles in both presynaptic Ca(2+) homeostasis and the modulation of H(+) gradient in SVs. |
