| First Author | Ono E | Year | 2007 |
| Journal | J Gen Virol | Volume | 88 |
| Issue | Pt 10 | Pages | 2636-41 |
| PubMed ID | 17872513 | Mgi Jnum | J:140284 |
| Mgi Id | MGI:3813202 | Doi | 10.1099/vir.0.83080-0 |
| Citation | Ono E, et al. (2007) Comparison of the antiviral potentials among the pseudorabies-resistant transgenes encoding different soluble forms of porcine nectin-1 in transgenic mice. J Gen Virol 88(Pt 10):2636-41 |
| abstractText | Nectin-1 is an alphaherpesvirus receptor that binds to virion glycoprotein D by the first immunoglobulin (Ig)-like domain. The possibility of making animals resistant to pseudorabies virus (PRV) infection has been investigated by generating transgenic mice expressing soluble forms of porcine nectin-1. Previously, transgenic mice were generated that expressed a fusion protein made of the entire ectodomain of nectin-1 fused to the Fc portion of human IgG, or the first Ig-like domain fused to the Fc portion of porcine IgG. Here, the contribution of the second and third Ig-like domains of nectin-1 was analysed by generating transgenic mice expressing the entire ectodomain of nectin-1 fused to the porcine Fc portion. Transgenic mice expressing each of three different fusion proteins were challenged with PRV for comparison of their resistance. Altogether, mice transgenic for a chimera that carried the entire ectodomain were more resistant than those transgenic for a chimera that carried the first Ig-like domain. |
