| First Author | Shirasawa T | Year | 1993 |
| Journal | EMBO J | Volume | 12 |
| Issue | 5 | Pages | 1827-34 |
| PubMed ID | 8491176 | Mgi Jnum | J:11860 |
| Mgi Id | MGI:60130 | Doi | 10.1002/j.1460-2075.1993.tb05831.x |
| Citation | Shirasawa T, et al. (1993) A novel gene product associated with mu chains in immature B cells. EMBO J 12(5):1827-34 |
| abstractText | A previously unreported B cell specific gene, which we have named 8HS-20, was isolated from the cDNA library of a pre-B cell clone by subtraction and differential hybridization. This gene is selectively expressed as a 0.75 kb transcript in pre-B and bone marrow-derived B cell lines; a transcript of the same size is also found in bone marrow and, albeit at low levels, in spleen. The deduced amino acid sequence of the 8HS-20 cDNA displayed homology to a B cell specific gene, VpreB-1, and to members of the immunoglobulin supergene family including V lambda, V kappa, VH, TCRV alpha, V beta and CD8. Biochemical analysis using purified antiserum against 8HS-20 oligopeptides indicates that the gene encodes proteins with mol. wts of 13.5, 14, 15.5 and 16 kDa, which associate with mu chains in pre-B cell lines, and that these molecules are expressed concomitantly with VpreB-1 and lambda 5 gene products in the same cell lines. |
