| Primary Identifier | MGI:5288517 | Allele Type | Transgenic |
| Attribute String | Knockdown | Gene | Tg(Rnu6-RNAi:Bccip)4Zshn |
| Strain of Origin | FVB/N | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | A knockdown vector was constructed with a conditional Rnu6 (U6) promoter (disrupted by insertion of a loxP-flanked neo cassette) driving short hairpin RNA specific for mouse Bccip. The construct was injected into FVB/N fertilized oocytes. Two founder lines (4 and 13) were generated. Line 4 was analyzed to a greater extent. Cre recombination will excise the floxed neo cassette, reconstitutng activity of the Rnu6 promoter and allowing transcription of the anti-Bccip shRNA resulting in RNA interference of the Bccip gene. |
