| First Author | Gao J | Year | 1996 |
| Journal | Biochem Biophys Res Commun | Volume | 218 |
| Issue | 1 | Pages | 292-7 |
| PubMed ID | 8573148 | Mgi Jnum | J:30656 |
| Mgi Id | MGI:78159 | Doi | 10.1006/bbrc.1996.0051 |
| Citation | Gao J, et al. (1996) Complete nucleotide sequence, structural organization, and an alternatively spliced exon of mouse h1-calponin gene. Biochem Biophys Res Commun 218(1):292-7 |
| abstractText | From a CC1.2 embryonic stem cell genomic library, we isolated and sequenced a 10.4-kb DNA segment (GenBank/EMBL Data Bank accession number L49022) containing the entire gene encoding mouse h1-calponin, an actin-associated smooth muscle-specific protein and a potential modulator of contraction. Sequence data revealed that there are seven exons and six introns in the h1-calponin gene. Determined by primer extension mapping of the RNA transcripts, the transcription of h1-calponin gene initiates at the same site in stomach, urinary bladder and pregnant uterus smooth muscles. The genomic organization suggests that the previously identified alpha- and beta-calponin isoforms are produced by splicing of exon 7 at two alternative acceptor sites. Isolation and structural characterization of the h1-calponin gene provides information to further investigate the expression regulation of this smooth muscle-specific gene. |
