Primary Identifier | MGI:6810180 | Allele Type | Targeted |
Attribute String | Conditional ready, Epitope tag | Gene | Ptpn22 |
Transmission | Germline | Strain of Origin | C57BL/6N |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Sequence for a loxP site, a OneâSTrEPâtag (OST) and a Gly-Ser-Gly spacer was inserted in-frame at the 5â² end of the gene coding sequence and a second loxP site in inton 1. The FRT site flanked neomycin resistance gene cassette that was also inserted was removed through subsequent Flp-mediated recombination. The fusion of the OST to the protein can be used in affinity purification. |