| First Author | Fahrenkrug SC | Year | 1999 |
| Journal | Biochem Biophys Res Commun | Volume | 264 |
| Issue | 3 | Pages | 662-7 |
| PubMed ID | 10543989 | Mgi Jnum | J:58393 |
| Mgi Id | MGI:1347439 | Doi | 10.1006/bbrc.1999.1558 |
| Citation | Fahrenkrug SC, et al. (1999) Genomic organization and genetic mapping of the bovine PREF-1 gene. Biochem Biophys Res Commun 264(3):662-7 |
| abstractText | As a potential regulator of nutrient partitioning in beef cattle, we have cloned and genetically mapped the bovine PREF-1 gene. A full-length PREF-1 cDNA was isolated by iterative purification from a mixed-tissue cDNA library to which adipose contributed mRNA. Analysis of partial cDNAs from this library revealed that the 3'-terminal exon of the bovine PREF-1 mRNA is spliced in a manner analogous to its murine ortholog. However, we have also detected a PREF-1 splice form apparently unique to cattle. Aside from this alternative selection of a splice donor in the bovine fifth exon, the exon/intron junctions of the bovine PREF-1 gene recapitulate those observed for mice. The sequences proximal to the bovine PREF-1 transcription start site are homologous to the mouse PREF-1 promoter. Importantly, the sequence experimentally identified as critical to PREF-1 'suppression in adipocyte differentiation' is conserved in the bovine gene. The bovine PREF-1 gene was mapped to the telomeric end of BTA 21 by virtue of a physically linked microsatellite with seven alleles and 285 informative meiosis. Copyright 1999 Academic Press. |
