| First Author | Zhuravleva K | Year | 2020 |
| Journal | Microvasc Res | Volume | 132 |
| Pages | 104067 | PubMed ID | 32877697 |
| Mgi Jnum | J:299889 | Mgi Id | MGI:6490783 |
| Doi | 10.1016/j.mvr.2020.104067 | Citation | Zhuravleva K, et al. (2020) The tight junction protein cingulin regulates the vascular response to burn injury in a mouse model. Microvasc Res 132:104067 |
| abstractText | Edema formation due to the collapse of physiological barriers and the associated delayed healing process is still a central problem in the treatment of burn injuries. In healthy individuals, tight junctions form a barrier to fluid and small molecules. Cingulin is a cytoplasmic component of tight junctions and is involved in the regulation of the paracellular barrier. Endothelial specific cingulin knock-out mice provide new insight into the influence of tight junction proteins on edema formation and angiogenesis during wound healing. Knock-out mice lacking the head domain of cingulin in endothelial cells (Cgn(DeltaEC)) were created by breeding Cgn(fl/fl) mice with Tie1-cre mice. Using a no-touch hot air jet a burn trauma was induced on the ear of the mouse. Over a period of 12 days microcirculatory parameters such as edema formation, angiogenesis and leukocyte-endothelial interactions were visualized using intravital fluorescence microscopy. At baseline, Cgn(DeltaEC) mice surprisingly showed significantly less tracer extravasation compared to Cgn(fl/fl) littermates, whereas, after burn injury, edema was consistently higher in Cgn(DeltaEC) mice. Non-perfused area after wounding was increased, but there was no difference in vessel diameters, contraction or dilation of arteries in Cgn(DeltaEC) mice. Moreover, cingulin knock-out did not cause a difference in leukocyte adhesion after burn injury. In summary, cingulin limits non-perfused area after burn injury and maintains the paracellular barrier of blood vessels. Since edema formation with serious systemic effects is a central problem of burn wounds, understanding the importance of tight junction proteins might help to find new treatment strategies for burn wounds. |
