Primary Identifier | MGI:4834846 | Allele Type | Targeted |
Gene | Hprt1 | Transmission | Germline |
Strain of Origin | (B6.129P2-Hprt1<b-m3>/J x 129S-Gt(ROSA)26Sor<tm1Sor>/J)F1 | Is Recombinase | false |
Is Wild Type | false | Project Collection | Pleiades Promoter Project |
description | Germ line transmission of mutant cell line mEMS4619 has been established. |
molecularNote | The Ple17-lacZ transgene (pEMS1487) was designed with the 3948 bp Ple17 minipromoter (C8ORF46-D; derived from a portion of the promoter of the C8ORF46 gene and three downstream putative regulatory elements from the RIKEN cDNA 3110035E14 (C8ORF46) gene) upstream of a frt-flanked beta-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome. |