| First Author | Mourkioti F | Year | 2008 |
| Journal | Genesis | Volume | 46 |
| Issue | 8 | Pages | 424-30 |
| PubMed ID | 18693277 | Mgi Jnum | J:140318 |
| Mgi Id | MGI:3813372 | Doi | 10.1002/dvg.20419 |
| Citation | Mourkioti F, et al. (2008) Analysis of CRE-mediated recombination driven by myosin light chain 1/3 regulatory elements in embryonic and adult skeletal muscle: a tool to study fiber specification. Genesis 46(8):424-30 |
| abstractText | An increasing number of genes have been implicated in skeletal muscle fiber diversity. To study the contribution of diverse genetic elements to the regulation of fiber-type composition, we generated a transgenic mouse in which CRE recombinase expression is driven by muscle-specific regulatory sequences of the myosin light chain 1/3 locus (MLC). Using ROSA26 conditional reporter mice, we detected expression of the MLC-Cre transgene starting from embryonic day 12.5 (E12.5). By E15, recombination was detected in all muscle-derived structures. Immunohistochemical analysis revealed CRE activity was restricted to fast-twitch (type II) and excluded from slow-twitch (type I) fibers of skeletal muscle. The MLC-Cre transgenic mouse can be used in conjunction with conditional alleles to study both developmental patterning and maintenance of fast fiber-type phenotypes. |
