| First Author | Gribaudo G | Year | 1992 |
| Journal | J Biol Regul Homeost Agents | Volume | 6 |
| Issue | 4 | Pages | 137-41 |
| PubMed ID | 1296455 | Mgi Jnum | J:17942 |
| Mgi Id | MGI:65965 | Citation | Gribaudo G, et al. (1992) Molecular cloning of interferon-gamma inducible genes from a murine pre-B cell leukemia. J Biol Regul Homeost Agents 6(4):137-41 |
| abstractText | In the present study, a pre-B cell leukemia L1210-C7, representing a very early stage of the B lineage, was used to characterize the molecular mechanisms exploited by IFN-gamma to modulate B cell activity. A cDNA library was prepared with poly (A)+ RNA from cells stimulated with IFN-gamma and three cDNAs clones complementary to IFN-gamma inducible mRNAs were isolated by differential screening. Of these, the 9.5 cDNA hybridized to a 2.4 kb mRNA not homologous with previously cloned IFN-gamma inducible mRNAs. Furthermore, when compared with RNAs obtained from cells of different origins (fibroblasts and T cells) the 9.5 mRNA appeared to be increased only in cells belonging to the B lineage. Taken as a whole, these results demonstrate that in leukemic pre-B cells IFN-gamma induces the expression of a gene that could be employed as specific cell activation marker. |
