| First Author | Williams KL | Year | 2003 |
| Journal | J Immunol | Volume | 170 |
| Issue | 5 | Pages | 2417-26 |
| PubMed ID | 12594265 | Mgi Jnum | J:82013 |
| Mgi Id | MGI:2450511 | Doi | 10.4049/jimmunol.170.5.2417 |
| Citation | Williams KL, et al. (2003) BATF transgenic mice reveal a role for activator protein-1 in NKT cell development. J Immunol 170(5):2417-26 |
| abstractText | The importance of regulated AP-1 activity during T cell development was assessed using transgenic mice overexpressing BATF, a basic leucine zipper transcription factor and an AP-1 inhibitor. BATF transgenic animals possess normal thymic cellularity and all major T cell subsets, but show impaired thymocyte proliferation in vitro and no induction of IL-2, IL-4, IL-5, IL-10, and IL-13 expression. Since NKT cells are largely responsible for cytokine production in the thymus, this population was examined by detection of the V alpha 14-J alpha 281 TCR, flow cytometry of NK1.1(+) TCR beta(+) cells, and analysis of cytokine production by heat-stable Ag(low) thymocytes and peripheral NKT cells stimulated in vivo. Results show a severe under-representation of NKT cells in BATF transgenic animals, providing the first evidence that the precise control of AP-1-mediated transcription is critical for the proper emergence of thymus-derived NKT cells in the mouse. |
