| First Author | García de Frutos P | Year | 1995 |
| Journal | Biochim Biophys Acta | Volume | 1261 |
| Issue | 2 | Pages | 285-9 |
| PubMed ID | 7711074 | Mgi Jnum | J:24250 |
| Mgi Id | MGI:71999 | Doi | 10.1016/0167-4781(95)00019-d |
| Citation | Garcia de Frutos P, et al. (1995) cDNA structure of rabbit C4b-binding protein alpha-chain. Preserved sequence motive in complement regulatory protein modules which bind C4b. Biochim Biophys Acta 1261(2):285-9 |
| abstractText | A full length cDNA clone for the alpha-chain of the rabbit complement regulatory protein C4b-binding protein (C4BP) was isolated from a liver cDNA library. The clone encoded an open reading frame of 597 amino acids, which included a signal peptide, eight short consensus repeats (SCR) and a carboxy terminal non-repeat region. Gel filtration of rabbit plasma and testing of fractions for factor I cofactor activity (C4BP-like) revealed two peaks of activity, the one with highest molecular weight corresponding in size to that of human C4b-binding protein. Comparison of the rabbit C4BP alpha-chain sequence with other SCR containing C3b/C4b binding proteins revealed highest sequence similarities between the second SCRs in C4BP from rabbit, human and murine species and SCRs at corresponding position in complement receptor 1 (CR1) whereas in decay accelerating factor (DAF), the third SCR was most similar. A conserved sequence motive was identified in these C4b-binding SCRs. |
