| First Author | Hu J | Year | 2009 |
| Journal | Cell | Volume | 138 |
| Issue | 3 | Pages | 549-61 |
| PubMed ID | 19665976 | Mgi Jnum | J:152713 |
| Mgi Id | MGI:4359556 | Doi | 10.1016/j.cell.2009.05.025 |
| Citation | Hu J, et al. (2009) A class of dynamin-like GTPases involved in the generation of the tubular ER network. Cell 138(3):549-61 |
| abstractText | The endoplasmic reticulum (ER) consists of tubules that are shaped by the reticulons and DP1/Yop1p, but how the tubules form an interconnected network is unknown. Here, we show that mammalian atlastins, which are dynamin-like, integral membrane GTPases, interact with the tubule-shaping proteins. The atlastins localize to the tubular ER and are required for proper network formation in vivo and in vitro. Depletion of the atlastins or overexpression of dominant-negative forms inhibits tubule interconnections. The Sey1p GTPase in S. cerevisiae is likely a functional ortholog of the atlastins; it shares the same signature motifs and membrane topology and interacts genetically and physically with the tubule-shaping proteins. Cells simultaneously lacking Sey1p and a tubule-shaping protein have ER morphology defects. These results indicate that formation of the tubular ER network depends on conserved dynamin-like GTPases. Since atlastin-1 mutations cause a common form of hereditary spastic paraplegia, we suggest ER-shaping defects as a neuropathogenic mechanism. |
