| First Author | Nakauchi J | Year | 2000 |
| Journal | Neurosci Lett | Volume | 287 |
| Issue | 3 | Pages | 231-5 |
| PubMed ID | 10863037 | Mgi Jnum | J:63544 |
| Mgi Id | MGI:1861159 | Doi | 10.1016/s0304-3940(00)01169-1 |
| Citation | Nakauchi J, et al. (2000) Cloning and characterization of a human brain Na(+)-independent transporter for small neutral amino acids that transports D-serine with high affinity. Neurosci Lett 287(3):231-5 |
| abstractText | We isolated a cDNA for the human homologue of system asc transporter Asc-1 from human brain. The encoded protein designated as hAsc-1 (human Asc-1) exhibited 91 % sequence identity to mouse Asc-1. Consistent with mouse Asc-1, hAsc-1 required 4F2 heavy chain for its functional expression in Xenopus oocytes. hAsc-1 exhibited the properties of amino acid transport system asc which transports small neutral amino acids in a Na(+)-independent manner. hAsc-1 transported D-serine at high affinity with a K(m) value of 22.8 &mgr;M. In brain, 2.0 kb mRNA was highly expressed. hAsc-1 gene was mapped to human chromosome 19, region q12-q13.1. Because of the high-affinity transport with the K(m) value close to the physiological concentration of D-serine, together with the high levels of expression in brain, hAsc-1 is proposed to play significant roles in the D-serine mobilization in brain. |
