| Primary Identifier | MGI:6469907 | Allele Type | Targeted |
| Attribute String | Not Specified | Gene | Nras |
| Transmission | Germline | Strain of Origin | C57BL/6 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The targeting vector is designed to insert an FRT-flanked neomycin resistance (neo) cassette downstream of exon 5. Endogenous exon 5 was modified to harbor a guanine to cytosine mutation at codon 181, resulting in a cysteine to serine mutation (C181S). This targeting vector then was used to retarget Nrastm1Tyj inserting C181S in cis to the existing G12Dmutation. Flp-mediated recombination removed the FRT-flanked neo cassette. |
