| First Author | Tian M | Year | 2013 |
| Journal | PLoS One | Volume | 8 |
| Issue | 3 | Pages | e58832 |
| PubMed ID | 23555598 | Mgi Jnum | J:200166 |
| Mgi Id | MGI:5507748 | Doi | 10.1371/journal.pone.0058832 |
| Citation | Tian M, et al. (2013) Light-induced translocation of RGS9-1 and Gbeta5L in mouse rod photoreceptors. PLoS One 8(3):e58832 |
| abstractText | The transducin GTPase-accelerating protein complex, which determines the photoresponse duration of photoreceptors, is composed of RGS9-1, Gbeta5L and R9AP. Here we report that RGS9-1 and Gbeta5L change their distribution in rods during light/dark adaptation. Upon prolonged dark adaptation, RGS9-1 and Gbeta5L are primarily located in rod inner segments. But very dim-light exposure quickly translocates them to the outer segments. In contrast, their anchor protein R9AP remains in the outer segment at all times. In the dark, Gbeta5L's interaction with R9AP decreases significantly and RGS9-1 is phosphorylated at S(475) to a significant degree. Dim light exposure leads to quick de-phosphorylation of RGS9-1. Furthermore, after prolonged dark adaptation, RGS9-1 and transducin Galpha are located in different cellular compartments. These results suggest a previously unappreciated mechanism by which prolonged dark adaptation leads to increased light sensitivity in rods by dissociating RGS9-1 from R9AP and redistributing it to rod inner segments. |
