Primary Identifier | MGI:3826396 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Clec2d |
Transmission | Germline | Strain of Origin | B6.Cg-Thy1<a> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP site was inserted upstream of exon 3 and a downstream floxed neo cassettes was inserted downstream of exon 3. Chimeric progeny were bred with a cre deleter strain to remove exon 3, leaving behind a single loxP site. Gene inactivation was confirmed by Southern blotting of cDNA derived from tail mRNA. Expression levels of Clec2g and Clec2i genes were unaffected in these mice. |