| First Author | Aström P | Year | 2014 |
| Journal | Exp Cell Res | Volume | 328 |
| Issue | 1 | Pages | 217-27 |
| PubMed ID | 25036555 | Mgi Jnum | J:217568 |
| Mgi Id | MGI:5614544 | Doi | 10.1016/j.yexcr.2014.07.010 |
| Citation | Astrom P, et al. (2014) Matrix metalloproteinase-8 regulates transforming growth factor-beta1 levels in mouse tongue wounds and fibroblasts in vitro. Exp Cell Res 328(1):217-27 |
| abstractText | Matrix metalloproteinase-8 (MMP-8)-deficient mice (Mmp8-/-) exhibit delayed dermal wound healing, but also partly contradicting results have been reported. Using the Mmp8-/- mice we investigated the role of MMP-8 in acute wound healing of the mobile tongue, and analyzed the function of tongue fibroblasts in vitro. Interestingly, in the early phase the tongue wounds of Mmp8-/- mice healed faster than those of wild type (wt) mice resulting in significant difference in wound widths (P=0.001, 6-24h). The Mmp8-/- wounds showed no change in myeloperoxidase positive myeloid cell count, but the level of transforming growth factor (TGF)-beta1 was significantly increased (P=0.007) compared to the wt tongues. Fibroblasts cultured from wt tongues expressed MMP-8 and TGF-beta1. However, higher TGF-beta1 levels were detected in Mmp8-/- fibroblasts, and MMP-8 treatment decreased phosphorylated Smad-2 levels and alpha-smooth muscle actin expression in these fibroblasts suggesting reduced TGF-beta1 signaling. Consistently, a degradation of recombinant TGF-beta1 by MMP-8 decreased its ability to activate the signaling cascade in fibroblasts. Moreover, collagen gels with Mmp8-/- fibroblasts reduced more in size. We conclude that MMP-8 regulates tongue wound contraction rate and TGF-beta1 levels. In vitro analyses suggest that MMP-8 may also play a role in regulating TGF-beta1 signaling of stromal fibroblasts. |
