| First Author | Lobito AA | Year | 2004 |
| Journal | Eur J Immunol | Volume | 34 |
| Issue | 3 | Pages | 890-898 |
| PubMed ID | 14991619 | Mgi Jnum | J:88389 |
| Mgi Id | MGI:3032905 | Doi | 10.1002/eji.200324675 |
| Citation | Lobito AA, et al. (2004) Ectopic T cell receptor expression causes B cell immunodeficiency in transgenic mice. Eur J Immunol 34(3):890-8 |
| abstractText | Mice expressing transgenic T cell receptors (TCR) are used to explore important questions in immunity. However, transgene expression may have unexpected effects. We previously reported a B cell immunodeficiency, comprising decreased B cell numbers and diminished antibody responses, in mice that express a transgenic TCR specific for nicotinic acetylcholine receptor; the mice were generated using cassette vectors designed specifically for transgenic TCR expression [see Kouskoff et al. J. Immunol. Methods 1995. 180: 273-280]. We now show data suggesting that this defect is due to the expression and accumulation of TCR alpha and beta chains inside B cells and induction of an endoplasmic reticulum stress response, causing apoptosis at the pre B-I and later B cell stage. Thus, inappropriate transgene expression can profoundly affect B cells, leading to a previously undescribed mechanism of immunodeficiency. |
