| First Author | Ban J | Year | 1998 |
| Journal | Biochem Biophys Res Commun | Volume | 248 |
| Issue | 1 | Pages | 147-52 |
| PubMed ID | 9675101 | Mgi Jnum | J:48559 |
| Mgi Id | MGI:1270955 | Doi | 10.1006/bbrc.1998.8907 |
| Citation | Ban J, et al. (1998) Identification of a human cDNA encoding a novel Bcl-x isoform. Biochem Biophys Res Commun 248(1):147-52 |
| abstractText | Alternative splicing has been shown to generate two isoforms of the apoptosis regulator bcl-x, Bcl-xL and Bcl-xS, in humans. Here we describe the identification and characterization of a third splice variant of the human bcl-x gene. It differs from previously described bcl-x transcripts in two respects: (1) a novel facultative intron is spliced out at the 5' untranslated region and (2) the open reading frame arises from a continuous genomic sequence extending over the splice donor sites utilized by the bcl-xL and bcl-xS transcripts. Since the resulting molecule has an organisation homologous to mouse and rat Bcl-x beta we suggest calling this novel protein human Bcl-x beta. Northern blot analysis revealed that bcl-x beta mRNA is expressed in numerous cell lines. Like Bcl-xL, h-Bcl-x binds to the pro-apoptotic protein Bax, suggesting a functional activity in vivo. |
