| First Author | Williams SC | Year | 1986 |
| Journal | Mol Cell Biol | Volume | 6 |
| Issue | 11 | Pages | 3807-14 |
| PubMed ID | 3796595 | Mgi Jnum | J:8538 |
| Mgi Id | MGI:57004 | Doi | 10.1128/mcb.6.11.3807 |
| Citation | Williams SC, et al. (1986) Mouse apolipoprotein A-IV gene: nucleotide sequence and induction by a high-lipid diet. Mol Cell Biol 6(11):3807-14 |
| abstractText | Apolipoprotein A-IV (apo A-IV) functions in conjunction with other apolipoproteins to form lipoprotein particles which are involved in lipid homeostasis. In this report we present the nucleotide sequence of the mouse apo A-IV gene and demonstrate its induction in the liver by chronically high dietary lipid. The apo A-IV gene consists of three exons and two introns. The introns separate evolutionarily conserved and functional polypeptide domains. Intron 1 divides most of the apo A-IV signal peptide from the amino terminus of the mature plasma protein. The second intron separates a highly evolutionarily conserved, variant amphipathic peptide repeat from the remainder of the mature apo A-IV protein. The 5' flanking region has several interesting features. The apo A-IV gene has variant TATA and CAT box sequences, TTTAAA and CCAACG, respectively. There are five G-rich direct repeats of 10 nucleotides and a short inverted repeat in the 5' flanking region. We speculate that these sequence elements in the 5' flanking region may be involved in the regulation of apo A-IV gene expression. We also show that chronically high dietary lipid induces liver apo A-IV levels 10-fold in C57BL/6 mice, a strain susceptible to atherosclerotic lesions, while we observed no induction in nonsusceptible BALB/c and C3H mice. |
