| First Author | Mischak H | Year | 1993 |
| Journal | FEBS Lett | Volume | 326 |
| Issue | 1-3 | Pages | 51-5 |
| PubMed ID | 8325388 | Mgi Jnum | J:12912 |
| Mgi Id | MGI:61128 | Doi | 10.1016/0014-5793(93)81759-s |
| Citation | Mischak H, et al. (1993) Unique expression pattern of protein kinase C-theta: high mRNA levels in normal mouse testes and in T-lymphocytic cells and neoplasms. FEBS Lett 326(1-3):51-5 |
| abstractText | A 2.2-kb cDNA that contains the entire coding region of mouse protein kinase C-theta (PKC-theta) was cloned from skeletal muscle mRNA using reverse transcription and the polymerase chain reaction (PCR). This clone was used as a probe to study the expression of this PKC isoform in normal and transformed hemopoietic cells and other normal tissues. By far the highest steady-state level of PKC-theta mRNA was found as a 2.8-kb transcript on a Northern blot of poly(A)+ RNA from testes. High levels were also found in skeletal muscle, spleen, T lymphomas and purified normal T lymphocytes, but these tissues and cells expressed two transcripts, 3.3 kb and 3.8 kb. Lower levels of similar size transcripts were found in normal brain, B lymphocytes and B-lymphocytic tumors and cell lines. |
