| Primary Identifier | MGI:2664398 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(DO11.10)10Dlo |
| Strain of Origin | (C57BL/6 x C3H)F1 x BALB/c | Is Recombinase | false |
| Is Wild Type | false |
| description | Mice carrying this pair of MHC class II restricted rearranged Tcra and Tcrb T cell receptor transgenes on a H2d background react to ovalbumin (OVA) peptide antigen. Intraperitoneal administration of OVA to transgenic mice results in a rapid deletion of the immature CD4+ CD8+ TCRlo thymocytes with progression to mature thymocytes. Apoptosis of cortical thymocytes in these mice occurs within 20 hours of treatment. |
| molecularNote | This double transgene contains rearranged Tcra and Tcrb genes and is expressed in the majority of T cells. These rearranged transgenes from the DO11.10 T cell hybridoma encode a chicken ovalbumin-specific MHC class II (I-Ad)-restricted T cell receptor. The allele was created by injection of a mix of the two separate transgenes Tg(TcraDO11.10)#Dlo and Tg(TcrbDO11.10)#Dlo into oocytes. |
