| First Author | Behrsing O | Year | 1992 |
| Journal | J Immunol Methods | Volume | 156 |
| Issue | 1 | Pages | 69-77 |
| PubMed ID | 1431164 | Mgi Jnum | J:844 |
| Mgi Id | MGI:49377 | Doi | 10.1016/0022-1759(92)90012-i |
| Citation | Behrsing O, et al. (1992) Bispecific IgA/IgM antibodies and their use in enzyme immunoassay. J Immunol Methods 156(1):69-77 |
| abstractText | Two hybrid hybridomas secreting polymeric bispecific antibodies to human chorionic gonadotropin and calf intestinal alkaline phosphatase were produced by fusion of IgA- and IgM-secreting mouse hybridomas. Both hybrid antibodies were purified from ascitic fluid by size exclusion chromatography. An IgM-like fraction was shown to exhibit bispecific activity. Bispecificity was completely lost following mild reduction and alkylation. Both bispecific antibodies were used to develop a sensitive enzyme immunoassay for hCG. |
